Simultaneous determination of p-aminohippuric acid, acetyl-paminohippuric acid and iothalamate in human plasma and urine by high-performance liquid chromatography
dc.contributor.author | Dowling, Thomas C. | |
dc.contributor.author | Frye, Reginald F. | |
dc.contributor.author | Zemaitis, Michael A. | |
dc.date.accessioned | 2012-01-10T14:20:34Z | |
dc.date.available | 2012-01-10T14:20:34Z | |
dc.date.issued | 1998 | |
dc.identifier.citation | Dowling, T. C., Frye, R. F., & Zemaitis, M. A. (1998). Simultaneous determination of p-aminohippuric acid, acetyl-paminohippuric acid and iothalamate in human plasma and urine by high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 716(1-2), 305-313, DOI: 10.1016/S0378-4347(98)00294-1. | en_US |
dc.identifier.issn | 0378-4347 | |
dc.identifier.uri | http://hdl.handle.net/10713/722 | |
dc.description.abstract | A sensitive and specific high-performance liquid chromatographic assay was developed for the simultaneous determination of p-aminohippuric acid (PAH), acetyl-p-aminohippuric acid (aPAH), and iothalamate in human plasma and urine. Plasma samples were prepared by protein precipitation with acetonitrile followed by evaporation, reconstitution in mobile phase, and injection onto a C reversed-phase column. Urine samples were diluted with 3 volumes of mobile phase prior to injection. 18 Column effluent was monitored by UV detection at 254 nm. The lower limits of quantification in plasma were 0.5 mg/ l for PAH and aPAH, and 1.0 mg/ l for iothalamate. The within-day and between-day coefficients of variation in plasma and urine were #7.8% for all analytes. This method is well suited for renal function studies using iothalamate and PAH, whether administered as a bolus dose or by continuous infusion, to measure glomerular filtration rate and effective renal plasma flow,respectively. | en_US |
dc.language.iso | en_US | en_US |
dc.subject | acetyl-p-aminohippuric acid | en_US |
dc.subject | iothalamate | en_US |
dc.subject.mesh | p-Aminohippuric Acid | en_US |
dc.subject.mesh | Iothalamic Acid | en_US |
dc.title | Simultaneous determination of p-aminohippuric acid, acetyl-paminohippuric acid and iothalamate in human plasma and urine by high-performance liquid chromatography | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/S0378-4347(98)00294-1 | |
dc.identifier.ispublished | Yes | en_US |