Now showing items 41-60 of 13012

    • Evaluation of different ways to identify persistent positivity of lupus anticoagulant in systemic lupus erythematosus

      Petri, Michelle A; Avci, Mertcan; Magder, Laurence S (BMJ Publishing Group, 2020-11-02)
      Among the 785 patients included in our analysis, the prevalence of persistent lupus anticoagulant as defined by the first two patient assessments was 4.3%. Annual assessment resulted in a prevalence of 6.6%, and using all 16 assessments resulted in a prevalence of 10.5%. The prevalence was substantially higher in men than in women, and in Caucasians than in African-Americans (p<0.01 for all comparisons). The rate of thrombosis was significantly elevated among those with persistently positive lupus anticoagulant by any definition (HR ranging from 2.75 to 3.42) relative to those without persistently positive lupus anticoagulant.
    • Prosthetic graft infection after vascular trauma

      Tchorz, Kathryn; Rozycki, Grace; Feliciano, David V (BMJ Publishing Group, 2020-11-03)
    • Alternative signaling pathways from IGF1 or insulin to AKT activation and FOXO1 nuclear efflux in adult skeletal muscle fibers

      Russell, Sarah J; Schneider, Martin F (American Society for Biochemistry and Molecular Biology Inc., 2020-08-31)
      Muscle atrophy is regulated by the balance between protein degradation and synthesis. FOXO1, a transcription factor, helps to determine this balance by activating pro-atrophic gene transcription when present in muscle fiber nuclei. Foxo1 nuclear efflux is promoted by AKT-mediated Foxo1 phosphorylation, eliminating FOXO1's atrophy-promoting effect. AKT activation can be promoted by insulin-like growth factor 1 (IGF1) or insulin via a pathway including IGF1 or insulin, phosphatidylinositol 3-kinase, and AKT. We used confocal fluorescence time-lapse imaging of FOXO1-GFP in adult isolated living muscle fibers maintained in culture to explore the effects of IGF1 and insulin on FOXO1-GFP nuclear efflux with and without pharmacological inhibitors. We observed that although AKT inhibitor blocks the IGF1- or insulin-induced effect on FOXO1 nuclear efflux, phosphatidylinositol 3-kinase inhibitors, which we show to be effective in these fibers, do not. We also found that inhibition of the protein kinase ACK1 or ATM contributes to the suppression of FOXO1 nuclear efflux after IGF1. These results indicate a novel pathway that has been unexplored in the IGF1- or insulin-induced regulation of FOXO1 and present information useful both for therapeutic interventions for muscle atrophy and for further investigative areas into insulin insensitivity and type 2 diabetes.
    • Comparison of transgenic and adenovirus hACE2 mouse models for SARS-CoV-2 infection

      Rathnasinghe, Raveen; Strohmeier, Shirin; Amanat, Fatima; Gillespie, Virginia L; Krammer, Florian; García-Sastre, Adolfo; Coughlan, Lynda; Schotsaert, Michael; Uccellini, Melissa B (Springer Nature, 2020-11-06)
      Severe acute respiratory syndrome CoV-2 (SARS-CoV-2) is currently causing a worldwide pandemic with high morbidity and mortality. Development of animal models that recapitulate important aspects of coronavirus disease 2019 (COVID-19) is critical for the evaluation of vaccines and antivirals, and understanding disease pathogenesis. SARS-CoV-2 has been shown to use the same entry receptor as SARS-CoV-1, human angiotensin-converting enzyme 2 (hACE2) [1-3]. Due to amino acid differences between murine and hACE2, inbred mouse strains fail to support high titer viral replication of SARS-CoV-2 virus. Therefore, a number of transgenic and knock-in mouse models, as well as viral vector-mediated hACE2 delivery systems have been developed. Here we compared the K18-hACE2 transgenic model to adenovirus-mediated delivery of hACE2 to the mouse lung. We show that K18-hACE2 mice replicate virus to high titers in the nasal turbinates, lung and brain, with high lethality, and cytokine/chemokine production. In contrast, adenovirus-mediated delivery results in viral replication to lower titers limited to the nasal turbinates and lung, and no clinical signs of infection. The K18-hACE2 model provides a stringent model for testing vaccines and antivirals, whereas the adenovirus delivery system has the flexibility to be used across multiple genetic backgrounds and modified mouse strains.
    • Financial incentives versus standard of care to improve patient compliance with live kidney donor follow-up: protocol for a multi-center, parallel-group randomized controlled trial

      Levan, Macey L; Waldram, Madeleine M; DiBrito, Sandra R; Thomas, Alvin G; Al Ammary, Fawaz; Ottman, Shane; Bannon, Jaclyn; Brennan, Daniel C; Massie, Allan B; Scalea, Joseph; et al. (Springer Nature, 2020-11-09)
      Background: Live kidney donors (LKDs) account for nearly a third of kidney transplants in the United States. While donor nephrectomy poses minimal post-surgical risk, LKDs face an elevated adjusted risk of developing chronic diseases such as hypertension, diabetes, and end-stage renal disease. Routine screening presents an opportunity for the early detection and management of chronic conditions. Transplant hospital reporting requirements mandate the submission of laboratory and clinical data at 6-months, 1-year, and 2-years after kidney donation, but less than 50% of hospitals are able to comply. Strategies to increase patient engagement in follow-up efforts while minimizing administrative burden are needed. We seek to evaluate the effectiveness of using small financial incentives to promote patient compliance with LKD follow-up. Methods/design: We are conducting a two-arm randomized controlled trial (RCT) of patients who undergo live donor nephrectomy at The Johns Hopkins Hospital Comprehensive Transplant Center (MDJH) and the University of Maryland Medical Center Transplant Center (MDUM). Eligible donors will be recruited in-person at their first post-surgical clinic visit or over the phone. We will use block randomization to assign LKDs to the intervention ($25 gift card at each follow-up visit) or control arm (current standard of care). Follow-up compliance will be tracked over time. The primary outcome will be complete (all components addressed) and timely (60 days before or after expected visit date), submission of LKD follow-up data at required 6-month, 1-year, and 2-year time points. The secondary outcome will be transplant hospital-level compliance with federal reporting requirements at each visit. Rates will be compared between the two arms following the intention-to-treat principle. Discussion: Small financial incentivization might increase patient compliance in the context of LKD follow-up, without placing undue administrative burden on transplant providers. The findings of this RCT will inform potential center- and national-level initiatives to provide all LKDs with small financial incentives to promote engagement with post-donation monitoring efforts. Trial registration: number: NCT03090646 Date of registration: March 2, 2017 Sponsors: Johns Hopkins University, University of Maryland Medical Center Funding: The Living Legacy Foundation of Maryland © 2020, The Author(s).
    • Successful transfer of anti-SARS-CoV-2 immunity using convalescent plasma in an MM patient with hypogammaglobulinemia and COVID-19

      Luetkens, Tim; Metcalf, Ryan; Planelles, Vicente; Zheng, Yue; Larragoite, Erin T; Spivak, Emily S; Spivak, Adam M; Steinbach, Mary; Blaylock, Robert C; Avila, Stephanie V; et al. (American Society of Hematology, 2020-10-08)
    • Incompletely Reported Important Methodological Details and Inaccurate Description of the Formulation That the Control Arms Received in a Gardasil Vaccine Trial

      Bourgeois, Florence; Doshi, Peter; Hong, Kyungwan; Jefferson, Tom; Jones, Mark; Lee, Haeyoung; Rowhani-Farid, Anisa; Shamseer, Larissa; Spence, O'Mareen (American Society for Microbiology, 2020-11-04)
    • nursingfor|um 2020

      University of Maryland, Baltimore. School of Nursing, 2020
    • SINI 2019: Powerful Partnerships

      Smith, Janis B.; Bergerhofer, Lacey (2019-07)
    • U.S. News & World Report: Master's Programs in Nursing - Peer Assessment Survey 2021

      University of Maryland, Baltimore. School of Nursing (2020)
    • American Association of Colleges of Nursing Survey 2020

      University of Maryland, Baltimore. School of Nursing (2020)
    • Disenfranchised Grief and Resilience Among Gay Widowers: A Phenomenological Exploration

      McNutt, Bryan R. (2014)
      Due to the continued prevalence of socio-cultural attitudes of sexual prejudice and stigma towards sexual minority relationships, and the continued lack of consistently inclusive legal protections for same-sex couples, bereaved gay widowers face considerable risk of encountering psychosocial features of disenfranchised grief at some point throughout their mourning process. In addition to providing a comprehensive review of previous research related to the bereavement experience of sexual minorities, this study considered the important role of psychological resilience for gay widowers in managing the bereavement recovery process and concurrent experiences of sexual minority stress. A descriptive phenomenological approach was applied through the use of semistructured in-depth interviews with five (5) gay widowers mourning the death of a same-sex partner due to a non-HIV/AIDS related cause. Results identified three primary constituents supporting a healthy trajectory of grief experience among gay widowers, while also providing indicators contributing to enhanced emotional and social resiliency: (1) Validation and Affirmation, (2) Family of Origin Integration, and (3) Positive Self- Regard. Likewise, the opposite constructs of these constituents (devaluation and disregard, family exclusion, and negative self-regard) also serve as likely indicators of increased vulnerability to developing complicated forms of disenfranchised grief, as well as difficulty accessing emotional and social resilience. Thus, the descriptions of these lived experiences provide further understanding of the influence of sexual minority stress upon the bereavement process of gay widowers, while also emphasizing the critical role of social validation and interpersonal recognition in promoting emotional resilience.