Iron is an essential micronutrient for bacterial growth and survival, yet its redox activity promotes the formation of reactive oxygen species (ROS), requiring organisms to tightly regulate iron uptake, utilization, and storage. Pseudomonads provide a rich system for examining these processes, as species within this genus occupy diverse ecological niches but share a strong dependence on iron metabolism. This dissertation investigates iron regulatory mechanisms in Pseudomonas aeruginosa and Pseudomonas fluorescens, with a particular focus on the iron-responsive PrrF small RNAs (sRNAs). These sRNAs are central to maintaining iron homeostasis, but their contributions to oxidative stress responses remain poorly defined. Using the PrrF sRNAs as a molecular probe, I examined how peroxide stress influences their expression and regulatory activity. My work provides new insight into the intersection of iron regulation and oxidative stress protection by demonstrating that hydrogen peroxide alters PrrF-mediated regulation in P. aeruginosa. I further extended this analysis to P. fluorescens, generating the first characterization of PrrF regulation by iron limitation and peroxide stress in a non-pathogenic pseudomonad. These findings reveal both conserved and divergent regulatory features that may reflect each species’ evolutionary adaptation to distinct environments. A major component of this thesis is an investigation of PA4880, a conserved Dps-like protein and PrrF target. In my thesis, I demonstrate that PA4880 expression is regulated by PrrF during iron limitation and this protein is induced by peroxide treatment. Preliminary findings also suggest a potential role for BrnD in biofilm formation, raising new questions about how iron and oxidative stress responses intersect with community-associated growth. Together, this work expands our understanding of iron homeostasis and oxidative stress regulation in the pseudomonads, laying the foundation for future studies exploring how these pathways evolve and contribute to survival across environmental niches.