OBJECTIVE: Effective endodontic treatment relies on root canal sealers' physicochemical properties, which fill irregularities and prevent bacterial infiltration. Traditional sealers exhibit limitations, while bioceramic-based sealers present enhanced properties that may improve clinical outcomes. This in vitro study compared the key physicochemical properties of four root canal sealers: NeoSEALER® Flo, EndoCeramic®, EndoSequence® BC Sealer, and ProRoot MTA®. METHODS: The sealer properties were evaluated according to ISO 6876:2012, including setting time, working time, flowability, dimensional stability, film thickness, and solubility. Statistical analyses were conducted using ANOVA with post hoc Tukey tests, triplicates included, and significance set at a P value less than 0.05. RESULTS: Significant variations in setting times were observed, with EndoSequence® BC Sealer having the longest (1428.67 min) and ProRoot MTA® the shortest (251.33 min) (p< 0.05). Dimensional stability tests revealed EndoCeramic® had minimal changes (0.48%) after 30 days, while NeoSEALER® Flo showed the most significant change (1.12%) (p< 0.05). Solubility results revealed significant differences, with NeoSEALER® Flo having the highest solubility (11.52%) and EndoCeramic® the lowest (2.08%) (p >0.05). Working time (min), flowability (mm), and film thickness (μm) were across sealers (p >0.05). CONCLUSION: EndoSequence® BC Sealer’s long setting time may delay clinical workflow, while ProRoot MTA®'s faster setting benefits time-sensitive treatments. EndoCeramic® offers high flowability and stability, with minimal dimensional changes, and is ideal for applications requiring precise sealer placement and long-term stability. NeoSEALER® Flo's high solubility may limit its longevity in clinical use where low solubility is desired.