Problem: Prompt recognition and treatment of neonatal sepsis are crucial to improve patient outcomes. Delays in antibiotic administration can lead to prolonged hospital stays, prolonged mechanical ventilation, and increased mortality. Pre-implementation data from a Level III NICU showed an average first-dose antibiotic administration time of 128 minutes, exceeding the recommended timeline by 68 minutes. Purpose: This Quality Improvement project aimed to reduce first-dose antibiotic administration times through a 15-week implementation of an antibiotic administration bundle. Methods: A visual reminder system was introduced to prompt staff to "start the clock" upon admission. An algorithm was developed to prioritize IV access, outlining steps for peripheral and umbilical access based on infant weight. If central access was obtained, the algorithm further described the process of obtaining blood cultures and administering antibiotics before confirming line placement via x-ray. Among 100 admitted infants, 44 received Ampicillin and were included in data collection. Results: Data showed 54% of infants received Ampicillin within 60 minutes of admission, with IV access established in 68% of infants within the same timeframe. The average administration time decreased by 55%, from 128 minutes to 70 minutes. Conclusion: An evidence-based algorithm demonstrated improved timeliness in first-dose antibiotic administration.