Lymphatic filariasis is a mosquito-borne filarial disease primarily occurring in the equatorial regions of Africa and Southeast Asia. The causative agents of this disease are three filarial parasites: Wuchereria bancrofti, Brugia malayi, and Brugia timori. B. malayi, and the related Brugia pahangi, are used to study this disease in the laboratory. A meta-analysis of publicly available B. malayi transcriptomic data identified protein-coding genes overlapping predicted rRNAs, a lack of nematode biological response to drug treatment, and established the consistency of the transcriptomic data over time as sequencing technology changes. With respect to lymphatic filarial infections, while male mammals have been documented to be more susceptible to infection, there is no effect of host sex on the transcriptome of B. pahangi nematodes isolated from intraperitoneally infected gerbils. B. pahangi nematodes reared in subcutaneously infected gerbils exhibited transcriptional variation, limiting the ability to reliably identify differentially expressed genes. A nearly complete genome assembly of Acanthocheilonema viteae, a non-human parasitic nematode, is also presented here. Assembled using long Oxford Nanopore reads, the genome assembly includes 5 autosomes, an X chromosome, and almost all telomeres. Lateral gene transfers in A. viteae were also identified between A. viteae and its mitochondria, as well as a Wolbachia endosymbiont.