This study examines the impact of hairstyle and hair color on the perceived professionalism of a Black female orthodontist, investigating whether Afrocentric hairstyles and unnatural hair colors influence perceptions of hygiene, carefulness, punctuality, and knowledgeability. A total of 268 participants completed an online survey, first answering demographic questions and then evaluating digitally manipulated images of the same Black female orthodontist with different hairstyles, including a bun, straight hair, curls, afros, braids, and dreadlocks, in black, brown, and blonde colors. Each image was displayed for eight seconds, after which participants rated the orthodontist on professional attributes using a validated Likert-scale instrument (strongly disagree to strongly agree). The results indicate that hairstyles aligning with Eurocentric beauty standards, such as a neatly tied bun or straight hair, received the highest ratings across all categories, particularly in natural black color. In contrast, Afrocentric hairstyles, especially afros and dreadlocks, received significantly lower professionalism ratings (p < 0.001) comparative to the black bun. Non-natural hair colors, particularly blonde, were also rated lower in professionalism and perceived competence (p < 0.05), especially when paired with Afrocentric styles. Gender and racial differences were observed in the evaluations. Male respondents assigned lower ratings to Afrocentric hairstyles, particularly in the category of knowledgeability of the orthodontist with the blonde afro (p = 0.044), and White participants giving significantly lower ratings to black dreadlocks and blonde afros compared to Black participants (p < 0.05). The findings highlight the persistence of implicit biases in orthodontic settings, where Eurocentric beauty standards often overshadow clinical expertise and professional credibility in shaping evaluations. Such biases may affect patient trust, treatment adherence, and career advancement opportunities for Black female orthodontists. Fostering a supportive and inclusive professional environment, where colleagues actively challenge appearance-based biases and advocate for equity, is essential for ensuring fairness within the field.