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dc.contributor.authorDriscoll, A.J.
dc.contributor.authorKnoll, M.D.
dc.contributor.authorHammitt, L.L.
dc.date.accessioned2019-07-15T16:16:56Z
dc.date.available2019-07-15T16:16:56Z
dc.date.issued2017
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85034736869&doi=10.1093%2fcid%2fcix101&partnerID=40&md5=8f7e39cec0b2810b583358ab59c98988
dc.identifier.urihttp://hdl.handle.net/10713/9992
dc.description.abstractBackground: Antibiotic exposure and specimen volume are known to affect pathogen detection by culture. Here we assess their effects on bacterial pathogen detection by both culture and polymerase chain reaction (PCR) in children. Methods: PERCH (Pneumonia Etiology Research for Child Health) is a case-control study of pneumonia in children aged 1–59 months investigating pathogens in blood, nasopharyngeal/oropharyngeal (NP/OP) swabs, and induced sputum by culture and PCR. Antibiotic exposure was ascertained by serum bioassay, and for cases, by a record of antibiotic treatment prior to specimen collection. Inoculated blood culture bottles were weighed to estimate volume. Results: Antibiotic exposure ranged by specimen type from 43.5% to 81.7% in 4223 cases and was detected in 2.3% of 4863 controls. Antibiotics were associated with a 45% reduction in blood culture yield and approximately 20% reduction in yield from induced sputum culture. Reduction in yield of Streptococcus pneumoniae from NP culture was approximately 30% in cases and approximately 32% in controls. Several bacteria had significant but marginal reductions (by 5%–7%) in detection by PCR in NP/OP swabs from both cases and controls, with the exception of S. pneumoniae in exposed controls, which was detected 25% less frequently compared to nonexposed controls. Bacterial detection in induced sputum by PCR decreased 7% for exposed compared to nonexposed cases. For every additional 1 mL of blood culture specimen collected, microbial yield increased 0.51% (95% confidence interval, 0.47%–0.54%), from 2% when volume was ≤1 mL to approximately 6% for ≥3 mL. Conclusions: Antibiotic exposure and blood culture volume affect detection of bacterial pathogens in children with pneumonia and should be accounted for in studies of etiology and in clinical management. Copyright The Author 2017.en_US
dc.description.urihttps://www.doi.org/10.1093/cid/cix101en_US
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofClinical Infectious Diseases
dc.subjectAntibiotic exposureen_US
dc.subjectBlood cultureen_US
dc.subjectChildrenen_US
dc.subjectPneumoniaen_US
dc.titleThe effect of antibiotic exposure and specimen volume on the detection of bacterial pathogens in children with pneumoniaen_US
dc.typeArticleen_US
dc.identifier.doi10.1093/cid/cix101
dc.identifier.pmid28575366


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