Effects of long-term water-aging on novel anti-biofilm and protein-repellent dental composite
JournalInternational Journal of Molecular Sciences
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AbstractThe aims of this study were to: (1) synthesize an anti-biofilm and protein-repellet dental composite by combining 2-methacryloyloxyethyl phosphorylcholine (MPC) with quaternary ammonium dimethylaminohexadecyl methacrylate (DMAHDM); and (2) evaluate the effects of water-aging for 180 days on protein resistance, bacteria-killing ability, and mechanical properties of MPC-DMAHDM composite. MPC and DMAHDM were added into a resin composite. Specimens were stored in distilled water at 37 °C for 1, 30, 90, and 180 days. Mechanical properties were measured in three-point flexure. Protein attachment onto the composite was evaluated by a micro bicinchoninic acid approach. An oral plaque microcosm biofilm model was employed to evaluate oral biofilm viability vs. water-aging time. Mechanical properties of the MPC-DMAHDM composite after 180-day immersion matched those of the commercial control composite. The composite with 3% MPC + 1.5% DMAHDM had much stronger resistance to protein adhesion than control (p < 0.05). MPC + DMAHDM achieved much stronger biofilm-eradicating effects than MPC or DMAHDM alone (p < 0.05). Biofilm colony-forming units on the 3% MPC + 1.5% DMAHDM composite were three orders of magnitude lower than commercial control. The protein-repellent and antibacterial effects were durable and showed no loss in water-aging from 1 to 180 days. The novel MPC-DMAHDM composite possessed strong and durable resistance to protein adhesion and potent bacteria-eradicating function, while matching the load-bearing ability of a commercial dental composite. The novel MPC-DMAHDM composite represents a promising means of suppressing oral plaque growth, acid production, and secondary caries. Copyright 2017 by the authors; licensee MDPI, Basel, Switzerland.
SponsorsThis study was supported by National Natural Science Foundation of China NSFC 81500879 (Ning Zhang), Beijing Municipal Science and Technology Z151100003915137 (Ning Zhang), Beijing Municipal Administration of Hospitals' Youth QML 20161501 (Ning Zhang), NSFC 81400540 (Ke Zhang), Beijing Municipal Administration of Hospitals' Youth QML20151401 (Ke Zhang), NIH R01 DE17974 (Hockin H. K. Xu), and a Seed Grant (Hockin H. K. Xu) from the University of Maryland School of Dentistry.
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85010078915&doi=10.3390%2fijms18010186&partnerID=40&md5=7180244a0c81bff5d98b5046d0a76ed5; http://hdl.handle.net/10713/9991