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dc.contributor.authorKang, M.-S.
dc.contributor.authorLim, H.-S.
dc.contributor.authorOh, J.-S.
dc.date.accessioned2019-07-15T16:12:17Z
dc.date.available2019-07-15T16:12:17Z
dc.date.issued2017
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85020319551&doi=10.1093%2ffemspd%2fftx009&partnerID=40&md5=13a367683383dfc310874511a588cb87
dc.identifier.urihttp://hdl.handle.net/10713/9989
dc.description.abstractThe increasing prevalence of methicillin-resistant Staphylococcus aureus has become a major public health threat. While lactobacilli were recently found useful in combating various pathogens, limited data exist on their therapeutic potential for S. aureus infections. The aim of this study was to determine whether Lactobacillus salivarius was able to produce bactericidal activities against S. aureus and to determine whether the inhibition was due to a generalized reduction in pH or due to secreted Lactobacillus product(s). We found an 8.6-log10 reduction of planktonic and a 6.3-log10 reduction of biofilm S. aureus. In contrast, the previously described anti-staphylococcal effects of L. fermentum only caused a 4.0-log10 reduction in planktonic S. aureus cells, with no effect on biofilm S. aureus cells. Killing of S. aureus was partially pH dependent, but independent of nutrient depletion. Cell-free supernatant that was pH neutralized and heat inactivated or proteinase K treated had significantly reduced killing of L. salivarius than with pH-neutralized supernatant alone. Proteomic analysis of the L. salivarius secretome identified a total of five secreted proteins including a LysM-containing peptidoglycan binding protein and a protein peptidase M23B. These proteins may represent potential novel anti-staphylococcal agents that could be effective against S. aureus biofilms. Copyright Crown 2017.en_US
dc.description.sponsorshipYvonne Achermann was supported by a 3-year fellowship grant by the Swiss National Science Foundation (SNF) (Switzerland, PBZHP3 141483), and a grant from the Swiss Foundation for Medical-Biological Grants (SSMBS) (Switzerland, P3MP3 148362/1).en_US
dc.description.urihttps://www.doi.org/10.1093/femspd/ftx009en_US
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofPathogens and Disease
dc.subjectAntibacterial activityen_US
dc.subjectBiofilmen_US
dc.subjectLactobacillus fermentumen_US
dc.subjectLactobacillus salivariusen_US
dc.subjectLysMen_US
dc.subjectStaphylococcus aureusen_US
dc.titleAntimicrobial activity of Lactobacillus salivarius and Lactobacillus fermentum against Staphylococcus aureusen_US
dc.typeArticleen_US
dc.identifier.doi10.1093/femspd/ftx009
dc.identifier.pmid28158586


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