Novel dental adhesive with biofilm-regulating and remineralization capabilities
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AbstractThe mechanical properties and anti-caries effect of a novel anti-caries adhesive containing poly (amidoamine) dendrimer (PAMAM) and dimethylaminododecyl methacrylate (DMADDM) were investigated for the first time. Microtensile bond strength and surface charge density were measured for the novel anti-caries adhesives. Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii were chosen to form three-species biofilms. Lactic acid assay, MTT (3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, exopolysaccharide staining and live/dead staining were performed to study anti-biofilm effect of the adhesive. The TaqMan realtime polymerase chain reaction was used to study the proportion change in three-species biofilms of different groups. The Scanning Electron Microscope (SEM) was used to observe the remineralization effect of PAMAM and DMADDM. The results showed that incorporating PAMAM and DMADDM into adhesive had no adverse effect on the dentin bond strength. The 1% PAMAM and 5% DMADDM adhesive group showed anti-biofilm properties and developed a healthier biofilm with a lower chance of inducing dental caries. Combination of 1% PAMAM and 5% DMADDM solution maintained remineralization capability on dentin, similar to that using 1% PAMAM alone. In conclusion, the adhesive containing PAMAM and DMADDM had strong antimicrobial properties and biological remineralization capabilities, and is promising for anti-caries clinical applications. Copyright 2017 by the authors.
SponsorsThis study was supported by the National Key Research and Development Program of China 2016YFC1102700 (X.Z.); the National Natural Science Foundation of China Grant 81372889 (L.C.), 81600858 (B.R.), 81372890 (M.F.) and 81430011 (X.Z.), the Open Fund of State Key Laboratory of Oral Diseases (2040305193002), the Recruitment Program for Young Professionals (M.F.), the Youth Grant of the Science and Technology Department of Sichuan Province, China 2017JQ0028 (L.C.), NIH R01 DE17974 (H.H.K.X.), the University of Maryland School of Dentistry Bridging Fund (H.H.K.X.) and the University of Maryland seed grant (H.H.K.X.).
Poly (amidoamine) dendrimer
Quaternary ammonium monomers
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85011632642&doi=10.3390%2fma10010026&partnerID=40&md5=0d987cc873889cc8720cc58d51b2d12c; http://hdl.handle.net/10713/9916