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dc.contributor.authorSanogo, M.
dc.contributor.authorKone, B.
dc.contributor.authorDiarra, B.
dc.date.accessioned2019-07-15T16:12:06Z
dc.date.available2019-07-15T16:12:06Z
dc.date.issued2017
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85013499550&doi=10.1016%2fj.cmi.2017.01.004&partnerID=40&md5=f4dcface26c48d9501c64fc7d5d2e1fb
dc.identifier.urihttp://hdl.handle.net/10713/9880
dc.description.abstractObjectives: In Mali early detection and treatment of multidrug-resistant tuberculosis (MDR-TB) are still challenging due to the cost, time and/or complexity associated with regular tests. Microscopic Observation Drug Susceptibility (MODS) is a low-cost assay validated by WHO in 2010. It is a liquid-culture-based assay to detect the ‘cording’ characteristic of Mycobacterium tuberculosis complex and to assess susceptibility to both isoniazid and rifampicin defining multidrug-resistant tuberculosis (MDR-TB). In this study we aimed to evaluate the performance of MODS as diagnostic tool compared with a validated method—Mycobacteria Growth Indicator Tube/Antimicrobial Susceptibility Testing/Streptomycin, Isoniazid, Rifampicin and Ethambutol (MGIT/AST/SIRE). Methods and Results: Between January 2010 and October 2015 we included 98 patients with suspected TB in an observational cohort study. The sensitivity and specificity of MODS assay for detecting TB were respectively 94.12% and 85.71% compared with the reference MGIT/7H11 culture, with a Cohen κ coefficient of 0.78 (95% CI 0.517–1.043). The median time to culture positivity for MODS assay and MGIT (plus interquartile range, IQR) was respectively 8 days (IQR 5–11) and 6 days (IQR 5–6). In detecting patients with MDR-TB, the sensitivity and specificity of MODS assay were respectively 100% and 95.92%. The positive predictive value and negative predictive value were, respectively, 66.7% and 100%. The median turnaround times for obtaining MDR-TB results using MODS assay and MGIT/AST/SIRE was respectively 9 days and 35 days. Hence, the MODS assay rapidly identifies MDR-TB in Mali compared with the MGIT/AST/SIRE. Conclusion As an easy, simple, fast and affordable method, the MODS assay could significantly improve the management of TB. Copyright 2017 European Society of Clinical Microbiology and Infectious Diseasesen_US
dc.description.urihttps://www.doi.org/10.1016/j.cmi.2017.01.004en_US
dc.language.isoen_USen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofClinical Microbiology and Infection
dc.subject7H11 cultureen_US
dc.subjectMalien_US
dc.subjectMicroscopic observation and drug susceptibilityen_US
dc.subjectMultidrug-resistant tuberculosisen_US
dc.subjectMycobacteria Growth Indicator Tube/Antimicrobial Susceptibility Testing/Sterptomycin, Isoniazid, Rifampicin and Ethambutolen_US
dc.subjectTuberculosisen_US
dc.titlePerformance of microscopic observation drug susceptibility for the rapid diagnosis of tuberculosis and detection of drug resistance in Bamako, Malien_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.cmi.2017.01.004
dc.identifier.pmid28110049


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