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    Molecular characterization of the human stomach microbiota in Gastric Cancer Patients

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    Author
    Yu, G.
    Torres, J.
    Hu, N.
    Date
    2017
    Journal
    Frontiers in Cellular and Infection Microbiology
    Publisher
    Frontiers Media S.A.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://www.doi.org/10.3389/fcimb.2017.00302
    Abstract
    Helicobacter pylori (Hp) is the primary cause of gastric cancer but we know little of its relative abundance and other microbes in the stomach, especially at the time of gastric cancer diagnosis. Here we characterized the taxonomic and derived functional profiles of gastric microbiota in two different sets of gastric cancer patients, and compared them with microbial profiles in other body sites. Paired non-malignant and tumor tissues were sampled from 160 gastric cancer patients with 80 from China and 80 from Mexico. The 16S rRNA gene V3-V4 region was sequenced using MiSeq platform for taxonomic profiles. PICRUSt was used to predict functional profiles. Human Microbiome Project was used for comparison. We showed that Hp is the most abundant member of gastric microbiota in both Chinese and Mexican samples (51 and 24%, respectively), followed by oral-associated bacteria. Taxonomic (phylum-level) profiles of stomach microbiota resembled oral microbiota, especially when the Helicobacter reads were removed. The functional profiles of stomach microbiota, however, were distinct from those found in other body sites and had higher inter-subject dissimilarity. Gastric microbiota composition did not differ by Hp colonization status or stomach anatomic sites, but did differ between paired non-malignant and tumor tissues in either Chinese or Mexican samples. Our study showed that Hp is the dominant member of the non-malignant gastric tissue microbiota in many gastric cancer patients. Our results provide insights on the gastric microbiota composition and function in gastric cancer patients, which may have important clinical implications. Copyright 2017 Yu, Torres, Hu, Medrano-Guzman, Herrera-Goepfert, Humphrys, Wang, Wang, Ding, Ravel, Taylor, Abnet and Goldstein.
    Keyword
    16S rRNA
    gastric cancer
    Helicobacter pylori
    KEGG modules
    microbiome
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027562611&doi=10.3389%2ffcimb.2017.00302&partnerID=40&md5=07252af7fb66298d2ac8d24369347b60; http://hdl.handle.net/10713/9875
    ae974a485f413a2113503eed53cd6c53
    10.3389/fcimb.2017.00302
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    UMB Open Access Articles 2017

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