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dc.contributor.authorHumphrys, Michael S.
dc.contributor.authorYu, G.
dc.contributor.authorPhillips, S.
dc.date.accessioned2019-07-15T16:01:12Z
dc.date.available2019-07-15T16:01:12Z
dc.date.issued2017
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85015835488&doi=10.1186%2fs40168-016-0226-6&partnerID=40&md5=bfe0f6c41e74c073d089a23f2cf63224
dc.identifier.urihttp://hdl.handle.net/10713/9871
dc.description.abstractBackground: The goal of the study was to investigate whether cigarette smoking alters oral and nasal microbial diversity, composition, and structure. Twenty-three current smokers and 20 never smokers were recruited. From each subject, nine samples including supra and subgingiva plaque scrapes, saliva, swabs from five soft oral tissue sites, and one nasal swab from both the anterior nares were collected. 16S rRNA V3-V4 region was sequenced for microbial profiles. Results: We found that alpha diversity was lower in smokers than in nonsmokers in the buccal mucosa, but in other sample sites, microbial diversity and composition were not significantly different by smoking status. Microbial profiles differed significantly among eight oral sites. Conclusions: This study investigates the effect of cigarette smoking on different sites of the oral cavity and shows a potential effect of cigarette smoking on the buccal mucosa microbiota. The marked heterogeneity of the oral microbial ecosystem that we found may contribute to the stability of the oral microbiota in most sites when facing environmental perturbations such as that caused by cigarette smoking. Copyright The Author(s) 2017.en_US
dc.description.sponsorshipThis work was supported by the Intramural Research Program of the National Cancer Institute at the National Institutes of Health.en_US
dc.description.urihttps://www.doi.org/10.1186/s40168-016-0226-6en_US
dc.language.isoen_USen_US
dc.publisherBioMed Central Ltd.en_US
dc.relation.ispartofMicrobiome
dc.subject16S rRNA and cigarette smokeen_US
dc.subjectMicrobiotaen_US
dc.subjectNasal cavityen_US
dc.subjectOral cavityen_US
dc.titleThe effect of cigarette smoking on the oral and nasal microbiotaen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/s40168-016-0226-6
dc.identifier.pmid28095925


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