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    Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments

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    Author
    Wintrode, Patrick L.
    Masson, Glenn R.
    Burke, John E.
    Journal
    Nature Methods
    Publisher
    Nature Publishing Group
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1038/s41592-019-0459-y
    Abstract
    Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.
    Sponsors
    This study was in part facilitated and funded by the EU COST Action BM1403 (Native Mass Spectrometry and Related Methods for Structural Biology).
    Keyword
    hydrogen-deuterium exchange mass spectrometry
    protein dynamics
    Biophysical Phenomena
    Protein Conformation
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/9865
    Collections
    UMB Open Access Articles 2019

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