Multicenter evaluation of the modified carbapenem inactivation method and the carba NP for detection of carbapenemase-producing pseudomonas aeruginosa and acinetobacter baumannii
Date
2018Journal
Journal of Clinical MicrobiologyPublisher
American Society for MicrobiologyType
Article
Metadata
Show full item recordAbstract
The purpose of this study was to develop the modified carbapenem inactivation method (mCIM) for the detection of carbapenemase-producing Pseudomonas aeruginosa (CP-PA) and carbapenemase-producing Acinetobacter baumannii (CP-AB) and perform a multicenter evaluation of the mCIM and Carba NP tests for these nonfermenters. Thirty P. aeruginosa and 30 A. baumannii isolates previously characterized by whole-genome sequencing from the CDC-FDA Antibiotic Resistance Isolate Bank were evaluated, including CP isolates (Ambler class A, B, and D), non-carbapenemase-producing (non-CP) carbapenem-resistant isolates, and carbapenem-susceptible isolates. Initial comparison of a 1-l versus 10-l loop inoculum for the mCIM was performed by two testing sites and showed that 10 l was required for reliable detection of carbapenemase production among P. aeruginosa and A. baumannii. Ten testing sites then evaluated the mCIM using a 10-l loop inoculum. Overall, the mean sensitivity and specificity of the mCIM for detection of CP-PA across all 10 sites were 98.0% (95% confidence interval [CI], 94.3 to 99.6; range, 86.7 to 100) and 95% (95% CI, 89.8 to 97.7; range, 93.3 to 100), whereas the mean sensitivity and specificity among CP-AB were 79.8% (95% CI, 74.0 to 84.9; range, 36.3 to 95.7) and 52.9% (95% CI, 40.6 to 64.9; range, 28.6 to 100), respectively. At three sites that evaluated the performance of the Carba NP test using the same set of isolates, the mean sensitivity and specificity of the Carba NP test were 97.8% (95% CI, 88.2 to 99.9; range, 93.3 to 100) and 97.8% (95% CI, 88.2 to 99.9; range, 93.3 to 100) for P. aeruginosa and 18.8% (95% CI, 10.4 to 30.1; range, 8.7 to 26.1) and 100% (95% CI, 83.9 to 100; range, 100) for A. baumannii. Overall, we found both the mCIM and the Carba NP test to be accurate for detection of carbapenemase production among P. aeruginosa isolates and less reliable for use with A. baumannii isolates. Copyright Copyright 2017 American Society for Microbiology. All Rights Reserved.Sponsors
Support for the statistical analysis from the National Center for Research Resources and the National Center for Advancing Translational Sciences (NCATS) of the National Institutes of Health through Grant Number 1UL1TR001079.Identifier to cite or link to this item
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040077227&doi=10.1128%2fJCM.01369-17&partnerID=40&md5=6872917c07583ff0defffaaec6a7ec76; http://hdl.handle.net/10713/9681ae974a485f413a2113503eed53cd6c53
10.1128/JCM.01369-17
Scopus Count
Collections
Related articles
- Carbapenemase Detection among Carbapenem-Resistant Glucose-Nonfermenting Gram-Negative Bacilli.
- Authors: Simner PJ, Opene BNA, Chambers KK, Naumann ME, Carroll KC, Tamma PD
- Issue date: 2017 Sep
- Carbapenem inactivation: a very affordable and highly specific method for phenotypic detection of carbapenemase-producing Pseudomonas aeruginosa isolates compared with other methods.
- Authors: Akhi MT, Khalili Y, Ghotaslou R, Kafil HS, Yousefi S, Nagili B, Goli HR
- Issue date: 2017 Jun
- Evaluation of the Carbapenem Inactivation Method for Detection of Carbapenemase-Producing Gram-Negative Bacteria in Comparison with the RAPIDEC CARBA NP.
- Authors: Aktaş E, Malkoçoğlu G, Otlu B, Çopur Çiçek A, Külah C, Cömert F, Sandallı C, Gürsoy NC, Erdemir D, Bulut ME
- Issue date: 2017 Jun
- Modified Carbapenem Inactivation Method for Phenotypic Detection of Carbapenemase Production among Enterobacteriaceae.
- Authors: Pierce VM, Simner PJ, Lonsway DR, Roe-Carpenter DE, Johnson JK, Brasso WB, Bobenchik AM, Lockett ZC, Charnot-Katsikas A, Ferraro MJ, Thomson RB Jr, Jenkins SG, Limbago BM, Das S
- Issue date: 2017 Aug
- Carbapenemase-Producing Carbapenem-Resistant Enterobacteriaceae from Bangkok, Thailand, and Their Detection by the Carba NP and Modified Carbapenem Inactivation Method Tests.
- Authors: Laolerd W, Akeda Y, Preeyanon L, Ratthawongjirakul P, Santanirand P
- Issue date: 2018 Sep