Utilization of dried blood spot specimens can expedite nationwide surveillance of HIV drug resistance in resource-limited settings
Date
2018Journal
PLoS ONEPublisher
Public Library of ScienceType
Article
Metadata
Show full item recordAbstract
Introduction Surveillance of HIV drug resistance (HIVDR) is crucial to ensuring the continued success of antiretroviral therapy (ART) programs. With the concern of reduced genotyping sensitivity of HIV on dried blood spots (DBS), DBS for HIVDR surveillance have been limited to ART-naïve populations. To investigate if DBS under certain conditions may also be a feasible sample type for HIVDR testing in ART patients, we piloted nationwide surveys for HIVDR among ART patients using DBS in two African countries with rapid scale-up of ART. Methods EDTA-venous blood was collected to prepare DBS from adult and pediatric ART patients receiving treatment during the previous 12-36 months. DBS were stored at ambient temperature for two weeks and then at -80C until shipment at ambient temperature to the WHO-designated Specialized HIVDR Laboratory at CDC in Atlanta. Viral load (VL) was determined using NucliSENS ® HIV-1 v2.0 kits; HIVDR genotyping was performed using the ATCC HIV-1 Drug Resistance Genotyping kits. Results DBS were collected from 1,368 and 1,202 ART patients; 244 and 255 these specimens had VL 1,000 copies/mL in Kenya and Tanzania, respectively. The overall genotyping rate of those DBS with VL 1,000 copies/mL was 93.0% (95% CI: 89.1%-95.6%) in Kenya and 91.8% (87.7%-94.6%) in Tanzania. The turnaround times for the HIVDR surveys from the time of collecting DBS to completing laboratory testing were 6.5 months and 9.3 months for the Kenya and Tanzania surveys, respectively. Conclusions The study demonstrates a favorable outcome of using DBS for nationwide surveillance of HIVDR in ART patients. Our results confirm that DBS collected and stored at ambient temperature for two weeks, and shipped with routine courier services are a reliable sample type for large-scale surveillance of acquired HIVDR. This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.Identifier to cite or link to this item
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053143137&doi=10.1371%2fjournal.pone.0203296&partnerID=40&md5=144ea9054c860abc2b862180efa64ec0; http://hdl.handle.net/10713/9673ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0203296
Scopus Count
Collections
Related articles
- Evaluation of dried blood spots collected on filter papers from three manufacturers stored at ambient temperature for application in HIV-1 drug resistance monitoring.
- Authors: Rottinghaus EK, Beard RS, Bile E, Modukanele M, Maruping M, Mine M, Nkengasong J, Yang C
- Issue date: 2014
- Dried blood spot specimens are a suitable alternative sample type for HIV-1 viral load measurement and drug resistance genotyping in patients receiving first-line antiretroviral therapy.
- Authors: Rottinghaus EK, Ugbena R, Diallo K, Bassey O, Azeez A, Devos J, Zhang G, Aberle-Grasse J, Nkengasong J, Yang C
- Issue date: 2012 Apr
- Field evaluation of dried blood spots for routine HIV-1 viral load and drug resistance monitoring in patients receiving antiretroviral therapy in Africa and Asia.
- Authors: Monleau M, Aghokeng AF, Eymard-Duvernay S, Dagnra A, Kania D, Ngo-Giang-Huong N, Touré-Kane C, Truong LX, Chaix ML, Delaporte E, Ayouba A, Peeters M, ANRS 12235 Study Group.
- Issue date: 2014 Feb
- Field study of dried blood spot specimens for HIV-1 drug resistance genotyping.
- Authors: Parry CM, Parkin N, Diallo K, Mwebaza S, Batamwita R, DeVos J, Bbosa N, Lyagoba F, Magambo B, Jordan MR, Downing R, Zhang G, Kaleebu P, Yang C, Bertagnolio S
- Issue date: 2014 Aug
- Comparison of Ahlstrom grade 226, Munktell TFN, and Whatman 903 filter papers for dried blood spot specimen collection and subsequent HIV-1 load and drug resistance genotyping analysis.
- Authors: Rottinghaus E, Bile E, Modukanele M, Maruping M, Mine M, Nkengasong J, Yang C
- Issue date: 2013 Jan