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    Molecular characterization of Vibrio cholerae responsible for cholera epidemics in Uganda by PCR, MLVA and WGS

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    Author
    Bwire, G.
    Sack, D.A.
    Almeida, M.
    Date
    2018
    Journal
    PLoS Neglected Tropical Diseases
    Publisher
    Public Library of Science
    Type
    Article
    
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    See at
    https://dx.doi.org/10.1371/journal.pntd.0006492
    Abstract
    Background: For almost 50 years sub-Saharan Africa, including Uganda, has experienced several outbreaks due to Vibrio cholerae. Our aim was to determine the genetic relatedness and spread of strains responsible for cholera outbreaks in Uganda. Methodology/Principal findings: Sixty-three V. cholerae isolates collected from outbreaks in Uganda between 2014 and 2016 were tested using multiplex polymerase chain reaction (PCR), multi-locus variable number of tandem repeat analysis (MLVA) and whole genome sequencing (WGS). Three closely related MLVA clonal complexes (CC) were identified: CC1, 32% (20/63); CC2, 40% (25/63) and CC3, 28% (18/63). Each CC contained isolates from a different WGS clade. These clades were contained in the third wave of the 7thcholera pandemic strain, two clades were contained in the transmission event (T)10 lineage and other in T13. Analysing the dates and genetic relatedness revealed that V. cholerae genetic lineages spread between districts within Uganda and across national borders. Conclusion: The V. cholerae strains showed local and regional transmission within Uganda and the East African region. To prevent, control and eliminate cholera, these countries should implement strong cross-border collaboration and regional coordination of preventive activities. Copyright 2018 Bwire et al. http://creativecommons.org/licenses/by/4.0/
    Keyword
    Africa South of the Sahara
    Uganda
    Vibrio cholerae--genetics
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85049372901&doi=10.1371%2fjournal.pntd.0006492&partnerID=40&md5=ce055b246d370f706d230fb0a9b8360b; http://hdl.handle.net/10713/9475
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pntd.0006492
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