PublisherAmerican Society for Microbiology
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AbstractWomen of reproductive age with a Lactobacillus-dominated vaginal microbiota have a reduced risk of acquiring and transmitting HIV and a vaginal pH of ~4 due to the presence of ~1% (wt/vol) lactic acid. While lactic acid has potent HIV virucidal activity in vitro, whether lactic acid present in the vaginal lumen inactivates HIV has not been investigated. Here we evaluated the anti-HIV-1 activity of native, minimally diluted cervicovaginal fluid obtained from women of reproductive age (n = 20) with vaginal microbiota dominated by Lactobacillus spp. Inhibition of HIVBa-L was significantly associated with the protonated form of lactic acid in cervicovaginal fluid. The HIVBa-L inhibitory activity observed in the <3-kDa acidic filtrate was similar to that of the corresponding untreated native cervicovaginal fluid as well as that of clarified neat cervicovaginal fluid subjected to protease digestion. These ex vivo studies indicate that protonated lactic acid is a major anti-HIV-1 metabolite present in acidic cervicovaginal fluid, suggesting a potential role in reducing HIV transmission by inactivating virus introduced or shed into the cervicovaginal lumen. Copyright 2018 Tyssen et al.
SponsorsThis work was supported by the National Health and Medical Research Council of Australia (NHMRC; project grants 1028294 and 1088564). G.T. was supported by the NHMRC senior research fellowship program (grants 543105 and 1117748), and J.A.H. and M.A. were supported by an Australian Postgraduate Award through Monash University. J.R. was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under award no. U19AI084044.
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85049513969&doi=10.1128%2fmSphere.00055-18&partnerID=40&md5=eacb39f67e729386e9d4dda5aedb16e9; http://hdl.handle.net/10713/9473
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