Exposure to concentrated ambient PM 2.5 compromises spermatogenesis in a mouse model: Role of suppression of hypothalamus-pituitary-gonads axis
PublisherOxford University Press
MetadataShow full item record
AbstractEpidemiological studies link ambient fine particulate matter (PM2.5) pollution to abnormalities in the male reproductive system. However, few toxicological studies have investigated this potentially important adverse effect of PM2.5 pollution. Therefore, in the present study, we analyzed the effects of PM2.5 exposure on spermatogenesis and hypothalamic-pituitary-gonadal (HPG) axis in a murine model. Fourteen male C57BL/6J mice were subjected to a 4-month exposure to filtered air or concentrated ambient PM2.5 (CAP). Their sperm count, testicular histology, spermatogenic parameters, and the major components of HPG axis were assessed. Exposure to CAP significantly reduced sperm count in the epididymis. This was accompanied by Sertoli cell vacuolization, immature germ cell dislocation, and decreases in pachytene spermatocytes and round spermatids of stage VII seminiferous tubules, suggesting a marked impairment of spermatogenesis in these mice. This impairment of spermatogenesis appeared to be attributable to a suppression of HPG axis subsequent to CAP exposure-induced hypothalamic inflammation, as exposure to CAP significantly increased TNFα and IL1b mRNA levels and meanwhile decreased gonadotropin-releasing hormone mRNA expression in the hypothalamus. Moreover, CAP exposure significantly reduced circulating testosterone and follicle-stimulating hormone, testicular testosterone and mRNA expression of follicle-stimulating hormone target gene SHBG and luteinizing hormone target genes P450scc, 17βHSD, and StAR. The present data demonstrate that exposure to ambient PM2.5 impairs spermatogenesis in murine model, raising the concern over effects of ambient PM2.5 pollution on the male reproductive function. Copyright The Author 2017. Published by Oxford University Press on behalf of the Society of Toxicology. All rights reserved.
SponsorsScience Foundation of China (Grant No. 81302452 to L.Q., 81270342 to Z.Y., 81500216 to M.C., 21407082 to X.W.), and Scientific Research Starting Foundation for The Doctoral Researcher of Nantong University (No. 14B14).
KeywordAmbient fine particulate matter (PM 2.5 )
Male reproductive system
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85049152275&doi=10.1093%2fTOXSCI%2fKFX261&partnerID=40&md5=8df463b392dc2e75a2c63d36e845fe63; http://hdl.handle.net/10713/9445
- Hypothalamic-pituitary-adrenal axis mediates ambient PM<sub>2.5</sub> exposure-induced pulmonary inflammation.
- Authors: Pan B, Chen M, Zhang X, Liang S, Qin X, Qiu L, Cao Q, Peng R, Tao S, Li Z, Zhu Y, Kan H, Xu Y, Ying Z
- Issue date: 2021 Jan 15
- Effect of cyanotoxins on the hypothalamic-pituitary-gonadal axis in male adult mouse.
- Authors: Xiong X, Zhong A, Xu H
- Issue date: 2014
- Adult exposure to bisphenol A (BPA) in Wistar rats reduces sperm quality with disruption of the hypothalamic-pituitary-testicular axis.
- Authors: Wisniewski P, Romano RM, Kizys MM, Oliveira KC, Kasamatsu T, Giannocco G, Chiamolera MI, Dias-da-Silva MR, Romano MA
- Issue date: 2015 Mar 2
- Concentrated ambient PM<sub>2.5</sub> exposure affects mice sperm quality and testosterone biosynthesis.
- Authors: Yang Y, Yang T, Liu S, Cao Z, Zhao Y, Su X, Liao Z, Teng X, Hua J
- Issue date: 2019
- The effects of recombinant FSH on testosterone-induced spermatogenesis in gonadotrophin-deficient (hpg) mice.
- Authors: Singh J, Handelsman DJ
- Issue date: 1996 Jul-Aug