• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles 2018
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles 2018
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Genomic analyses reveal the common occurrence and complexity of plasmodium vivax relapses in cambodia

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Author
    Popovici, J.
    Friedrich, L.R.
    Kim, S.
    Date
    2018
    Journal
    mBio
    Publisher
    American Society for Microbiology
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://dx.doi.org/10.1128/mBio.01888-17
    Abstract
    Plasmodium vivax parasites have a unique dormant stage that can cause relapses weeks or months after the initial infection. These dormant parasites are among the main challenges of vivax malaria control as they constitute a reservoir that is difficult to eliminate. Since field studies are confounded by reinfections and possible recrudescence of drug-resistant parasites, most analyses of P. vivax relapses have focused on travelers returning from regions of malaria endemicity. However, it is not clear whether these individuals accurately recapitulate the relapse patterns of repeatedly infected individuals residing in areas of endemicity. Here, we present analyses of vivax malaria patients enrolled in a tightly controlled field study in Cambodia. After antimalarial drug treatment was administered, we relocated 20 individuals to a nontransmission area and followed them for 60 days, with blood collection performed every second day. Our analyses reveal that 60% of the patients relapsed during the monitoring period. Using whole-genome sequencing and high-throughput genotyping, we showed that relapses in Cambodia are often polyclonal and that the relapsing parasites harbor various degrees of relatedness to the parasites present in the initial infection. Our analyses also showed that clone populations differed dynamically, with new clones emerging during the course of the relapsing infections. Overall, our study data show that it is possible to investigate the patterns, dynamics, and diversity of P. vivax relapses of individuals living in a region of malaria endemicity and reveal that P. vivax relapses are much more pervasive and complex than previously considered. (This study has been registered at Clinical-Trials.gov under registration no. NCT02118090.) IMPORTANCE P. vivax parasites can remain dormant in the liver and relapse weeks or months after the initial infection, greatly complicating malaria control and elimination efforts. The few investigations of this dormant stage have relied on travelers and military personnel returning from areas of malaria endemicity. However, it is not clear whether these individuals, exposed to a limited number of infections, accurately represent the patterns of relapses of individuals living in areas of endemicity, who are repeatedly infected by P. vivax parasites. Our study combined tightly controlled fieldwork with comprehensive genomic analyses, and our report provides a first opportunity to investigate the patterns, dynamics, and diversity of P. vivax relapses directly with individuals living in areas of endemicity. Copyright 2018 Popovici et al.
    Sponsors
    This work was funded by a National Institutes of Health-NIAID award to D.S. (R01 AI103228). L.R.F. was partially supported by a Case Western Reserve University training grant in geographic medicine (T32 A107024). Additional support was provided by a grant from the Institut Pasteur to D.M. (PTR 2014-490).
    Keyword
    Genomics
    Malaria
    Plasmodium vivax
    Relapse
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85043483707&doi=10.1128%2fmBio.01888-17&partnerID=40&md5=89bed346d68c5208004f0ca45f738379; http://hdl.handle.net/10713/9443
    ae974a485f413a2113503eed53cd6c53
    10.1128/mBio.01888-17
    Scopus Count
    Collections
    UMB Open Access Articles 2018

    entitlement

    Related articles

    • Exploration of Plasmodium vivax transmission dynamics and recurrent infections in the Peruvian Amazon using whole genome sequencing.
    • Authors: Cowell AN, Valdivia HO, Bishop DK, Winzeler EA
    • Issue date: 2018 Jul 4
    • Recrudescence, Reinfection, or Relapse? A More Rigorous Framework to Assess Chloroquine Efficacy for Plasmodium vivax Malaria.
    • Authors: Popovici J, Pierce-Friedrich L, Kim S, Bin S, Run V, Lek D, Hee KHD, Lee Soon-U L, Cannon MV, Serre D, Menard D
    • Issue date: 2019 Jan 7
    • A high resolution case study of a patient with recurrent Plasmodium vivax infections shows that relapses were caused by meiotic siblings.
    • Authors: Bright AT, Manary MJ, Tewhey R, Arango EM, Wang T, Schork NJ, Yanow SK, Winzeler EA
    • Issue date: 2014 Jun
    • Complexity of Infection and Genetic Diversity in Cambodian Plasmodium vivax.
    • Authors: Friedrich LR, Popovici J, Kim S, Dysoley L, Zimmerman PA, Menard D, Serre D
    • Issue date: 2016 Mar
    • Genotype comparison of Plasmodium vivax and Plasmodium falciparum clones from pregnant and non-pregnant populations in North-west Colombia.
    • Authors: Arango EM, Samuel R, Agudelo OM, Carmona-Fonseca J, Maestre A, Yanow SK
    • Issue date: 2012 Nov 26
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.