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dc.contributor.authorSengyee, S.
dc.contributor.authorYoon, S.H.
dc.contributor.authorPaksanont, S.
dc.date.accessioned2019-06-05T18:28:17Z
dc.date.available2019-06-05T18:28:17Z
dc.date.issued2018
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85044286618&doi=10.1371%2fjournal.pntd.0006287&partnerID=40&md5=a2d79a1a99ab3ffe946c0e7757cd1807
dc.identifier.urihttp://hdl.handle.net/10713/9432
dc.description.abstractBurkholderia pseudomallei is an environmental bacterium that causes melioidosis, a major community-acquired infection in tropical regions. Melioidosis presents with a range of clinical symptoms, is often characterized by a robust inflammatory response, may relapse after treatment, and results in high mortality rates. Lipopolysaccharide (LPS) of B. pseudomallei is a potent immunostimulatory molecule comprised of lipid A, core, and O-polysaccharide (OPS) components. Four B. pseudomallei LPS types have been described based on SDS-PAGE patterns that represent the difference of OPS–type A, type B, type B2 and rough LPS. The majority of B. pseudomallei isolates are type A. We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) followed by electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QqTOF MS) and gas chromatography to characterize the lipid A of B. pseudomallei within LPS type A isolates. We determined that B. pseudomallei lipid A is represented by penta- and tetra-acylated species modified with 4-amino-4-deoxy-arabinose (Ara4N). The MALDI-TOF profiles from 171 clinical B. pseudomallei isolates, including 68 paired primary and relapse isolates and 35 within-host isolates were similar. We did not observe lipid A structural changes when the bacteria were cultured in different growth conditions. Dose-dependent NF-κB activation in HEK cells expressing TLR4 was observed using multiple heat-killed B. pseudomallei isolates and corresponding purified LPS. We demonstrated that TLR4-dependent NF-κB activation induced by heat-killed bacteria or LPS prepared from OPS deficient mutant was significantly greater than those induced by wild type B. pseudomallei. These findings suggest that the structure of B. pseudomallei lipid A is highly conserved in a wide variety of clinical and environmental circumstances but that the presence of OPS may modulate LPS-driven innate immune responses in melioidosis. Copyright 2018 Sengyee et al.en_US
dc.description.urihttps://dx.doi.org/10.1371/journal.pntd.0006287en_US
dc.language.isoen-USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS Neglected Tropical Diseases
dc.subject.meshBurkholderia pseudomallei--immunologyen_US
dc.subject.meshLipid Aen_US
dc.subject.meshO Antigensen_US
dc.titleComprehensive analysis of clinical Burkholderia pseudomallei isolates demonstrates conservation of unique lipid A structure and TLR4-dependent innate immune activationen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pntd.0006287
dc.identifier.pmid29474381


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