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dc.contributor.authorHumeniuk, Piotr
dc.contributor.authorGeiselhart, Sabine
dc.contributor.authorBattin, Claire
dc.creatorHumeniuk, P.
dc.date.accessioned2019-05-28T15:14:57Z
dc.date.available2019-05-28T15:14:57Z
dc.date.issued2019-04-23
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85065767249&origin=inward
dc.identifier.urihttp://hdl.handle.net/10713/9327
dc.description.abstractInvariant natural killer T (iNKT) cells are a specialized subset of T cells contributing to both, the innate and adaptive immune responses. In contrast to conventional T lymphocytes they recognize lipid antigens. The aim of the project is to establish a novel model system, to study iNKT-TCR – ligand interaction. An iNKT reporter cell line (JE6-1 REP-iNKT ) was engineered by introducing the human iNKT-TCR into a human leukemic T cell line carrying an NF-κB-driven fluorescent transcriptional reporter construct. Antigen presenting BW STIM cells expressing human CD1d and CD80 were generated. Reporter induction in JE6-1 REP-iNKT cells was assessed by flow cytometry. CRISPR/Cas9 was used for β2M knock out in JE6-1 REP-iNKT cells to abrogate CD1d expression and thus excluding antigen self-presentation. Reporter cells were shown to specifically react with iNKT antigens presented via CD1d. Their sensitivity towards α-GalCer was comparable to a murine iNKT hybridoma cell line. In conclusion, we created a novel iNKT reporter platform which, compared to traditional iNKT cell assays, is characterized by a shorter turnaround time and lower costs. It thus facilitates the identification of antigenic structures that drive the activation of iNKT cells in health and disease. © 2019, The Author(s).en_US
dc.description.sponsorshipTis work was partially funded by the Austrian Science Fund (FWF): DK W 1248-B13 for PH and SFB F4603 for KHS.en_US
dc.description.urihttps://dx.doi.org/10.1038/s41598-019-43529-4en_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofScientific Reportsen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.meshJurkat Cellsen_US
dc.subject.meshNatural Killer T-Cellsen_US
dc.titleGeneration of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptoren_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41598-019-43529-4
dc.relation.volume9


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Attribution 3.0 United States
Except where otherwise noted, this item's license is described as Attribution 3.0 United States