Show simple item record

dc.contributor.authorDjapgne, L.
dc.contributor.authorPanja, S.
dc.contributor.authorBrewer, L.K.
dc.date.accessioned2019-05-21T18:56:22Z
dc.date.available2019-05-21T18:56:22Z
dc.date.issued2018
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85045881384&doi=10.1128%2fJB.00704-17&partnerID=40&md5=4acb74292b6be8cde46e135680cd5ff2
dc.identifier.urihttp://hdl.handle.net/10713/9245
dc.description.abstractPseudomonas aeruginosa is an opportunistic Gram-negative pathogen that requires iron for growth and virulence. Under low-iron conditions, P. aeruginosa transcribes two highly identical (95%) small regulatory RNAs (sRNAs), PrrF1 and PrrF2, which are required for virulence in acute murine lung infection models. The PrrF sRNAs promote the production of 2-akyl-4(1H)-quinolone metabolites (AQs) that mediate a range of biological activities, including quorum sensing and polymicrobial interactions. Here, we show that the PrrF1 and PrrF2 sRNAs promote AQ production by redundantly inhibiting translation of antR, which encodes a transcriptional activator of the anthranilate degradation genes. A combination of genetic and biophysical analyses was used to define the sequence requirements for PrrF regulation of antR, demonstrating that the PrrF sRNAs interact with the antR 5= untranslated region (UTR) at sequences overlapping the translational start site of this mRNA. The P. aeruginosa Hfq protein interacted with UArich sequences in both PrrF sRNAs (Kd [dissociation constant] = 50 nM and 70 nM). Hfq bound with lower affinity to the antR mRNA (0.3 ?M), and PrrF was able to bind to antR mRNA in the absence of Hfq. Nevertheless, Hfq increased the rate of PrrF annealing to the antR UTR by 10-fold. These studies provide a mechanistic description of how the PrrF1 and PrrF2 sRNAs mediate virulence traits, such as AQ production, in P. aeruginosa. Copyright 2018 American Society for Microbiology.en_US
dc.description.sponsorshipThis work was supported by NIH grants R01 AI123320 (to A.G.O.-S., S.A.W., and M.A.K.) and R01 GM120425 (to S.A.W.) and a University of Maryland School of Pharmacy Merit Award Fellowship (to L.D.)en_US
dc.description.urihttps://dx.doi.org/10.1128/JB.00704-17en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofJournal of Bacteriology
dc.subjectAntRen_US
dc.subjectHfqen_US
dc.subjectIronen_US
dc.subjectPQSen_US
dc.subjectPrrFen_US
dc.subjectPseudomonas aeruginosaen_US
dc.titleThe Pseudomonas aeruginosa PrrF1 and PrrF2 small regulatory RNAs promote 2-alkyl-4- quinolone production through redundant regulation of the antR mRNAen_US
dc.typeArticleen_US
dc.identifier.doi10.1128/JB.00704-17
dc.identifier.pmid29507088


This item appears in the following Collection(s)

Show simple item record