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    The Pseudomonas aeruginosa PrrF1 and PrrF2 small regulatory RNAs promote 2-alkyl-4- quinolone production through redundant regulation of the antR mRNA

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    Author
    Djapgne, L.
    Panja, S.
    Brewer, L.K.
    Date
    2018
    Journal
    Journal of Bacteriology
    Publisher
    American Society for Microbiology
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://dx.doi.org/10.1128/JB.00704-17
    Abstract
    Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen that requires iron for growth and virulence. Under low-iron conditions, P. aeruginosa transcribes two highly identical (95%) small regulatory RNAs (sRNAs), PrrF1 and PrrF2, which are required for virulence in acute murine lung infection models. The PrrF sRNAs promote the production of 2-akyl-4(1H)-quinolone metabolites (AQs) that mediate a range of biological activities, including quorum sensing and polymicrobial interactions. Here, we show that the PrrF1 and PrrF2 sRNAs promote AQ production by redundantly inhibiting translation of antR, which encodes a transcriptional activator of the anthranilate degradation genes. A combination of genetic and biophysical analyses was used to define the sequence requirements for PrrF regulation of antR, demonstrating that the PrrF sRNAs interact with the antR 5= untranslated region (UTR) at sequences overlapping the translational start site of this mRNA. The P. aeruginosa Hfq protein interacted with UArich sequences in both PrrF sRNAs (Kd [dissociation constant] = 50 nM and 70 nM). Hfq bound with lower affinity to the antR mRNA (0.3 ?M), and PrrF was able to bind to antR mRNA in the absence of Hfq. Nevertheless, Hfq increased the rate of PrrF annealing to the antR UTR by 10-fold. These studies provide a mechanistic description of how the PrrF1 and PrrF2 sRNAs mediate virulence traits, such as AQ production, in P. aeruginosa. Copyright 2018 American Society for Microbiology.
    Sponsors
    This work was supported by NIH grants R01 AI123320 (to A.G.O.-S., S.A.W., and M.A.K.) and R01 GM120425 (to S.A.W.) and a University of Maryland School of Pharmacy Merit Award Fellowship (to L.D.)
    Keyword
    AntR
    Hfq
    Iron
    PQS
    PrrF
    Pseudomonas aeruginosa
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045881384&doi=10.1128%2fJB.00704-17&partnerID=40&md5=4acb74292b6be8cde46e135680cd5ff2; http://hdl.handle.net/10713/9245
    ae974a485f413a2113503eed53cd6c53
    10.1128/JB.00704-17
    Scopus Count
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    UMB Open Access Articles 2018

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