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dc.contributor.authorSantaus, T.M.
dc.contributor.authorLi, S.
dc.contributor.authorLadd, P.
dc.date.accessioned2019-05-21T18:56:20Z
dc.date.available2019-05-21T18:56:20Z
dc.date.issued2018
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85050657200&doi=10.1371%2fjournal.pone.0201070&partnerID=40&md5=592854338a4cd435924a63142d8a73ad
dc.identifier.urihttp://hdl.handle.net/10713/9229
dc.description.abstractSample preparation is a leading bottleneck in rapid detection of pathogenic bacteria. Here, we use Lyse-It® for bacterial cellular lysis, genomic DNA fragmentation, and protein release and degradation for both Listeria monocytogenes and Vibrio cholerae. The concept of Lyse-It® employs a conventional microwave and Lyse-It® slides for intensely focused microwave irradiation onto the sample. High microwave power and a <60 second irradiation time allow for rapid cellular lysis and subsequent intracellular component release. The pathogenic bacteria are identified by quantitative polymerase chain reaction (qPCR), which subsequently demonstrates the viability of DNA for amplification post microwave-induced lysis. Intracellular component release, degradation, and detection of L. monocytogenes and V. cholerae has been performed and shown in this paper. These results demonstrate a rapid, low-cost, and efficient way for bacterial sample preparation on both food and water-borne Gram-positive and -negative organisms alike. Copyright 2018 Santaus et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.description.urihttps://dx.doi.org/10.1371/journal.pone.0201070en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS ONE
dc.subjectcell lysisen_US
dc.subjectLyse-It®en_US
dc.subjectquantitative polymerase chain reactionen_US
dc.subject.meshBacteriological Techniques--methodsen_US
dc.subject.meshMicrowavesen_US
dc.titleRapid sample preparation with Lyse-It® for Listeria monocytogenes and Vibrio choleraeen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0201070
dc.identifier.pmid30044836


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