MetadataShow full item record
AbstractFörster resonance energy transfer (FRET) between fluorophores of the same species was recognized in the early to mid-1900s, well before modern heterotransfer applications. Recently, homotransfer FRET principles have re-emerged in biosensors that incorporate genetically encoded fluorescent proteins. Homotransfer offers distinct advantages over the standard heterotransfer FRET method, some of which are related to the use of fluorescence polarization microscopy to quantify FRET between two fluorophores of identical color. These include enhanced signal-to-noise, greater compatibility with other optical sensors and modulators, and new design strategies based upon the clustering or dimerization of singly-labeled sensors. Here, we discuss the theoretical basis for measuring homotransfer using polarization microscopy, procedures for data collection and processing, and we review the existing genetically-encoded homotransfer biosensors. Copyright 2018 by the authors.
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85054898147&doi=10.3390%2fbios8040089&partnerID=40&md5=726e46aae975a214a015447636d194ca; http://hdl.handle.net/10713/9210
- Triple Fluorescence Anisotropy Reporter Imaging in Living Cells.
- Authors: Ross B, Wong SH, Snell NE, Zhang J, Rizzo MA
- Issue date: 2019 May 5
- Development of FRET biosensors for mammalian and plant systems.
- Authors: Hamers D, van Voorst Vader L, Borst JW, Goedhart J
- Issue date: 2014 Mar
- Imaging of Genetically Encoded FRET-Based Biosensors to Detect GPCR Activity.
- Authors: Bordes L, Chavez-Abiega S, Goedhart J
- Issue date: 2021
- Fluorescent proteins for FRET microscopy: monitoring protein interactions in living cells.
- Authors: Day RN, Davidson MW
- Issue date: 2012 May
- Genetically encoded FRET-based biosensors for multiparameter fluorescence imaging.
- Authors: Carlson HJ, Campbell RE
- Issue date: 2009 Feb