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dc.contributor.authorWhite, K.
dc.contributor.authorYang, P.
dc.contributor.authorLi, L.
dc.date.accessioned2019-05-17T13:21:14Z
dc.date.available2019-05-17T13:21:14Z
dc.date.issued2018
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85045422178&doi=10.1089%2fbio.2017.0099&partnerID=40&md5=a5cce826ee8e507b3357e94e5f9c14d4
dc.identifier.urihttp://hdl.handle.net/10713/9163
dc.description.abstractBrain tissue from 1068 donors was analyzed for RNA quality as a function of postmortem interval (PMI) and years in storage. Approximately 83% of the cortical and cerebellar samples had an RNA integrity number (RIN) of 6 or greater, indicating their likely suitability for real-time quantitative polymerase chain reaction research. The average RIN value was independent of the PMI, up to at least 36 hours. The RNA quality for specific donated brains could not be predicted based on the PMI. Individual samples with a low PMI could have a poor RIN value, while a sample with a PMI over 36 hours may have a high RIN value. The RIN values for control brain donors, all of whom died suddenly and unexpectedly, were marginally higher than for individuals with clinical brain disorders. Polymerase chain reaction (PCR) analysis of samples confirmed that RIN values were more critical than PMI for determining suitability of tissue for molecular biological studies and samples should be matched by their RIN values rather than PMI. Importantly, PCR analysis established that tissue stored up to 23 years at −80°C yielded high-quality RNA. These results confirm that postmortem human brain tissue collected by brain and tissue banks over decades can serve as high quality material for the study of human disorders. Copyright Kimberly White et al. 2018.en_US
dc.description.sponsorshipThe generosity of the families of the tissue donors makes possible the wide range of basic medical studies that the UMD-BTB has supported with the hope of curing the many diseases affecting humanity. The Bank is supported by NIH contract No. HHSN275200900011C, Ref. No. NO1-HD-9-0011 with additional support from the Autism Research Institute and the Blazeman Foundation for ALS.en_US
dc.description.urihttps://dx.doi.org/10.1089/bio.2017.0099en_US
dc.language.isoen_USen_US
dc.publisherMary Ann Liebert Inc.en_US
dc.relation.ispartofBiopreservation and Biobanking
dc.subjectbrain banken_US
dc.subjecthuman brainen_US
dc.subjectlong-term storageen_US
dc.subjectPMIen_US
dc.subjectRNA qualityen_US
dc.titleEffect of Postmortem Interval and Years in Storage on RNA Quality of Tissue at a Repository of the NIH NeuroBioBanken_US
dc.typeArticleen_US
dc.identifier.doi10.1089/bio.2017.0099
dc.identifier.pmid29498539


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