Effect of Postmortem Interval and Years in Storage on RNA Quality of Tissue at a Repository of the NIH NeuroBioBank
JournalBiopreservation and Biobanking
PublisherMary Ann Liebert Inc.
MetadataShow full item record
AbstractBrain tissue from 1068 donors was analyzed for RNA quality as a function of postmortem interval (PMI) and years in storage. Approximately 83% of the cortical and cerebellar samples had an RNA integrity number (RIN) of 6 or greater, indicating their likely suitability for real-time quantitative polymerase chain reaction research. The average RIN value was independent of the PMI, up to at least 36 hours. The RNA quality for specific donated brains could not be predicted based on the PMI. Individual samples with a low PMI could have a poor RIN value, while a sample with a PMI over 36 hours may have a high RIN value. The RIN values for control brain donors, all of whom died suddenly and unexpectedly, were marginally higher than for individuals with clinical brain disorders. Polymerase chain reaction (PCR) analysis of samples confirmed that RIN values were more critical than PMI for determining suitability of tissue for molecular biological studies and samples should be matched by their RIN values rather than PMI. Importantly, PCR analysis established that tissue stored up to 23 years at −80°C yielded high-quality RNA. These results confirm that postmortem human brain tissue collected by brain and tissue banks over decades can serve as high quality material for the study of human disorders. Copyright Kimberly White et al. 2018.
SponsorsThe generosity of the families of the tissue donors makes possible the wide range of basic medical studies that the UMD-BTB has supported with the hope of curing the many diseases affecting humanity. The Bank is supported by NIH contract No. HHSN275200900011C, Ref. No. NO1-HD-9-0011 with additional support from the Autism Research Institute and the Blazeman Foundation for ALS.
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85045422178&doi=10.1089%2fbio.2017.0099&partnerID=40&md5=a5cce826ee8e507b3357e94e5f9c14d4; http://hdl.handle.net/10713/9163