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dc.contributor.authorOtt, J.A.
dc.contributor.authorCastro, C.D.
dc.contributor.authorDeiss, T.C.
dc.date.accessioned2019-05-17T13:21:14Z
dc.date.available2019-05-17T13:21:14Z
dc.date.issued2018-04-17
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85050694223&doi=10.7554%2feLife.28477&partnerID=40&md5=f9ac96420039ed1f8dee194a3929cf2c
dc.identifier.urihttp://hdl.handle.net/10713/9162
dc.description.abstractSince the discovery of the T cell receptor (TcR), immunologists have assigned somatic hypermutation (SHM) as a mechanism employed solely by B cells to diversify their antigen receptors. Remarkably, we found SHM acting in the thymus on α chain locus of shark TcR. SHM in developing shark T cells likely is catalyzed by activation-induced cytidine deaminase (AID) and results in both point and tandem mutations that accumulate non-conservative amino acid replacements within complementarity-determining regions (CDRs). Mutation frequency at TcRα was as high as that seen at B cell receptor loci (BcR) in sharks and mammals, and the mechanism of SHM shares unique characteristics first detected at shark BcR loci. Additionally, fluorescence in situ hybridization showed the strongest AID expression in thymic corticomedullary junction and medulla. We suggest that TcRα utilizes SHM to broaden diversification of the primary αβ T cell repertoire in sharks, the first reported use in vertebrates. Copyright Ott et al.en_US
dc.description.sponsorshipNational Science Foundationen_US
dc.description.urihttps://dx.doi.org/10.7554/eLife.28477en_US
dc.language.isoen_USen_US
dc.publishereLife Sciences Publications Ltden_US
dc.relation.ispartofeLife
dc.subjectnurse sharken_US
dc.subjectsomatic hypermutationen_US
dc.subject.meshGenes, T-Cell Receptor alphaen_US
dc.titleSomatic hypermutation of T cell receptor α chain contributes to selection in nurse shark thymusen_US
dc.title.alternativeSomatic hypermutation of T cell receptor alpha chain contributes to selection in nurse shark thymusen_US
dc.typeArticleen_US
dc.identifier.doi10.7554/eLife.28477
dc.identifier.pmid29664399


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