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    Somatic hypermutation of T cell receptor α chain contributes to selection in nurse shark thymus

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    Author
    Ott, J.A.
    Castro, C.D.
    Deiss, T.C.
    Date
    2018
    Journal
    eLife
    Publisher
    eLife Sciences Publications Ltd
    Type
    Article
    
    Metadata
    Show full item record
    Other Titles
    Somatic hypermutation of T cell receptor α chain contributes to selection in nurse shark thymus
    See at
    https://dx.doi.org/10.7554/eLife.28477
    Abstract
    Since the discovery of the T cell receptor (TcR), immunologists have assigned somatic hypermutation (SHM) as a mechanism employed solely by B cells to diversify their antigen receptors. Remarkably, we found SHM acting in the thymus on α chain locus of shark TcR. SHM in developing shark T cells likely is catalyzed by activation-induced cytidine deaminase (AID) and results in both point and tandem mutations that accumulate non-conservative amino acid replacements within complementarity-determining regions (CDRs). Mutation frequency at TcRα was as high as that seen at B cell receptor loci (BcR) in sharks and mammals, and the mechanism of SHM shares unique characteristics first detected at shark BcR loci. Additionally, fluorescence in situ hybridization showed the strongest AID expression in thymic corticomedullary junction and medulla. We suggest that TcRα utilizes SHM to broaden diversification of the primary αβ T cell repertoire in sharks, the first reported use in vertebrates. Copyright Ott et al.
    Sponsors
    National Science Foundation
    Keyword
    nurse shark
    somatic hypermutation
    Genes, T-Cell Receptor alpha
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050694223&doi=10.7554%2feLife.28477&partnerID=40&md5=f9ac96420039ed1f8dee194a3929cf2c; http://hdl.handle.net/10713/9162
    ae974a485f413a2113503eed53cd6c53
    10.7554/eLife.28477
    Scopus Count
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    UMB Open Access Articles 2018

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