Evaluation of α-tubulin, detyrosinated α-tubulin, and vimentin in CTCs: Identification of the interaction between CTCs and blood cells through cytoskeletal elements
JournalBreast Cancer Research
PublisherBioMed Central Ltd.
MetadataShow full item record
Other TitlesEvaluation of alpha-tubulin, detyrosinated alpha-tubulin, and vimentin in CTCs: Identification of the interaction between CTCs and blood cells through cytoskeletal elements
AbstractBackground: Circulating tumor cells (CTCs) are the major players in the metastatic process. A potential mechanism of cell migration and invasion is the formation of microtentacles in tumor cells. These structures are supported by ?-tubulin (TUB), detyrosinated ?-tubulin (GLU), and vimentin (VIM). In the current study, we evaluated the expression of those cytoskeletal proteins in CTCs. Methods: Forty patients with breast cancer (BC) (16 early and 24 metastatic) were enrolled in the study. CTCs were isolated using the ISET platform and stained with the following combinations of antibodies: pancytokeratin (CK)/VIM/TUB and CK/VIM/GLU. Samples were analyzed with the ARIOL platform and confocal laser scanning microscopy. Results: Fluorescence quantification revealed that the ratios CK/TUB, CK/VIM, and CK/GLU were statistically increased in MCF7 compared with more aggressive cell lines (SKBR3 and MDA-MB-231). In addition, all of these ratios were statistically increased in MCF7 cells compared with metastatic BC patients' CTCs (p = 0.0001, p = 0.0001, and p = 0.003, respectively). Interestingly, intercellular connections among CTCs and between CTCs and blood cells through cytoskeleton bridges were revealed, whereas microtentacles were increased in patients with CTC clusters. These intercellular connections were supported by TUB, VIM, and GLU. Quantification of the examined molecules revealed that the median intensity of TUB, GLU, and VIM was significantly increased in patients with metastatic BC compared with those with early disease (TUB, 62.27 vs 11.5, p = 0.0001; GLU, 6.99 vs 5.29, p = 0.029; and VIM, 8.24 vs 5.38, p = 0.0001, respectively). Conclusions: CTCs from patients with BC aggregate to each other and to blood cells through cytoskeletal protrusions, supported by VIM, TUB, and GLU. Quantification of these molecules could potentially identify CTCs related to more aggressive disease. Copyright 2018 The Author(s).
SponsorsThe authors acknowledge the partial support of this work by the Hellenic Oncology Research Group (HORG).
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85049586443&doi=10.1186%2fs13058-018-0993-z&partnerID=40&md5=c3fb2626aac0e689535455a3a2230f61; http://hdl.handle.net/10713/9151
- Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.
- Authors: Katsarou SD, Messaritakis I, Voumvouraki A, Kakavogiannis S, Κotsakis A, Alkahtani S, Stournaras C, Martin SS, Georgoulias V, Kallergi G
- Issue date: 2022 Jan 25
- Variable expression levels of keratin and vimentin reveal differential EMT status of circulating tumor cells and correlation with clinical characteristics and outcome of patients with metastatic breast cancer.
- Authors: Polioudaki H, Agelaki S, Chiotaki R, Politaki E, Mavroudis D, Matikas A, Georgoulias V, Theodoropoulos PA
- Issue date: 2015 May 13
- Epithelial to mesenchymal transition markers expressed in circulating tumour cells of early and metastatic breast cancer patients.
- Authors: Kallergi G, Papadaki MA, Politaki E, Mavroudis D, Georgoulias V, Agelaki S
- Issue date: 2011 Jun 10
- Analogous detection of circulating tumor cells using the AccuCyte<sup>®</sup> -CyteFinder<sup>®</sup> system and ISET system in patients with locally advanced and metastatic prostate cancer.
- Authors: van der Toom EE, Groot VP, Glavaris SA, Gemenetzis G, Chalfin HJ, Wood LD, Wolfgang CL, de la Rosette JJMCH, de Reijke TM, Pienta KJ
- Issue date: 2018 Mar
- Vimentin filaments support extension of tubulin-based microtentacles in detached breast tumor cells.
- Authors: Whipple RA, Balzer EM, Cho EH, Matrone MA, Yoon JR, Martin SS
- Issue date: 2008 Jul 15
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