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dc.contributor.authorVeDepo, M.
dc.contributor.authorBuse, E.
dc.contributor.authorQuinn, R.
dc.date.accessioned2019-05-17T12:53:04Z
dc.date.available2019-05-17T12:53:04Z
dc.date.issued2018
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85060662630&doi=10.1177%2f2041731418767216&partnerID=40&md5=3f22aeccff7acd1a83a31966f1035315
dc.identifier.urihttp://hdl.handle.net/10713/9087
dc.description.abstractThe tissue-engineered heart valve may be the ideal valve replacement option but still must overcome challenges in leaflet recellularization. This study sought to investigate the potential for leaflet matrix restoration and repopulation following mononuclear cell seeding and extended periods of bioreactor conditioning. Human aortic heart valves were seeded with mononuclear cells and conditioned in a pulsatile bioreactor for 3 days, 3 weeks, or 6 weeks. The results of this study determined that a mononuclear cell population can be readily localized within the leaflet tissue in as little as 3 days. Furthermore, as extended bioreactor condition continued to the 3- and 6-week time points, the mesenchymal stem cell subfraction proliferated and appeared to become the predominant cell phenotype. This was evident through positive expression of mesenchymal stem cell markers and no expression of mononuclear cell markers observed by immunohistochemistry in the 3- and 6-week groups. In addition, cells in the 3- and 6-week groups exhibited an up-regulation of mesenchymal stem cell-associated genes (THY1, NT5E, and ITGB1) and a down-regulation of mononuclear cell-associated genes (CD14, ICAM1, and PECAM1) compared to the initial seeded cell population. However, repopulation of the leaflet interstitium was less extensive than anticipated. Valves in the 6-week time point also exhibited retracted leaflets. Thus, while the 3-week bioreactor-conditioning period used in this study may hold some promise, a bioreactor-conditioning period of 6 weeks is not a viable option for clinical translation due to the negative impact on valve performance. Copyright The Author(s) 2018.en_US
dc.description.urihttps://dx.doi.org/10.1177/2041731418767216en_US
dc.language.isoen_USen_US
dc.publisherSAGE Publications Ltden_US
dc.relation.ispartofJournal of Tissue Engineering
dc.subjectbioreactor conditioningen_US
dc.subjectmononuclear cellsen_US
dc.subjectTissue-engineered heart valveen_US
dc.titleExtended bioreactor conditioning of mononuclear cell-seeded heart valve scaffoldsen_US
dc.typeArticleen_US
dc.identifier.doi10.1177/2041731418767216


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