Preserving prion strain identity upon replication of prions in vitro using recombinant prion protein
Date
2018Journal
Acta neuropathologica communicationsPublisher
BMCType
Article
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Last decade witnessed an enormous progress in generating authentic infectious prions or PrPSc in vitro using recombinant prion protein (rPrP). Previous work established that rPrP that lacks posttranslational modification is able to support replication of highly infectious PrPSc with assistance of cofactors of polyanionic nature and/or lipids. Unexpectedly, previous studies also revealed that seeding of rPrP by brain-derived PrPSc gave rise to new prion strains with new disease phenotypes documenting loss of a strain identity upon replication in rPrP substrate. Up to now, it remains unclear whether prion strain identity can be preserved upon replication in rPrP. The current study reports that faithful replication of hamster strain SSLOW could be achieved in vitro using rPrP as a substrate. We found that a mixture of phosphatidylethanolamine (PE) and synthetic nucleic acid polyA was sufficient for stable replication of hamster brain-derived SSLOW PrPSc in serial Protein Misfolding Cyclic Amplification (sPMCA) that uses hamster rPrP as a substrate. The disease phenotype generated in hamsters upon transmission of recombinant PrPSc produced in vitro was strikingly similar to the original SSLOW diseases phenotype with respect to the incubation time to disease, as well as clinical, neuropathological and biochemical features. Infrared microspectroscopy (IR-MSP) indicated that PrPSc produced in animals upon transmission of recombinant PrPSc is structurally similar if not identical to the original SSLOW PrPSc. The current study is the first to demonstrate that rPrP can support replication of brain-derived PrPSc while preserving its strain identity. In addition, the current work is the first to document that successful propagation of a hamster strain could be achieved in vitro using hamster rPrP.Identifier to cite or link to this item
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062022587&doi=10.1186%2fs40478-018-0597-y&partnerID=40&md5=2bf4c1377f7124915290dca591d57bb9; http://hdl.handle.net/10713/9058ae974a485f413a2113503eed53cd6c53
10.1186/s40478-018-0597-y
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Correction to: preserving prion strain identity upon replication of prions in vitro using recombinant prion protein (Acta neuropathologica communications (2018) 6 1 (92))Makarava, N.; Savtchenko, R.; Lasch, P. (BMC, 2018)Figure 6 of the original publication [1] contained an error in the Wavenumber in panels B and C. The wavenumbers 1616 (Cm-1) in panels B and C should have been 1516 (cm-1). The updated figure has been published in this correction article; the original article has been updated.
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