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    Complex effects of cytochrome P450 monooxygenase on purple membrane and bacterioruberin production in an extremely Halophilic archaeon: Genetic, phenotypic, and transcriptomic analyses

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    Author
    Müller, W.J.
    Smit, M.S.
    van Heerden, E.
    Date
    2018
    Journal
    Frontiers in Microbiology
    Publisher
    Frontiers Media S.A.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://dx.doi.org/10.3389/fmicb.2018.02563
    Abstract
    Halophilic archaea are known to produce a diverse array of pigments for phototrophy and photoprotection. The aim of this paper was to determine the role of a Halobacterium gene encoding the predicted cytochrome P450 monooxygenase (CYP174A1) in pigment synthesis through a combined genetic, phenotypic, and transcriptomic approach. We report on the observed phenotype changes [increased bacterioruberin levels and the loss of purple membrane (PM)] between the Halobacterium salinarum R1 and its CYP174A1-deletion mutant. In addition, we report on the whole-genome DNA microarray analysis, which supports the phenotype of PM loss. This work expands our understanding of the bop-gene regulon, and its relation to carotenoid biosynthesis, and sheds light on our broader understanding of the role (s) of CYP174A1 in archaeal pigment synthesis. To date, this is the first study in which the physiological role of any cytochrome P450 monooxygenase (CYP450) in extremely halophilic archaea has been reported. Copyright 2018 Frontiers Media S.A. All rights reserved.
    Sponsors
    The authors wish to thank the DasSarma laboratory for their useful inputs and the National Research Foundation (South Africa) for funding.
    Keyword
    Bacterioopsin
    Bacterioruberin
    Cytochrome P450
    Halophilic archaea
    Purple membrane (PM)
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85055829796&doi=10.3389%2ffmicb.2018.02563&partnerID=40&md5=a251df8b52d8edfa83808cf6557e74ba; http://hdl.handle.net/10713/9053
    ae974a485f413a2113503eed53cd6c53
    10.3389/fmicb.2018.02563
    Scopus Count
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