• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    PGC-1α regulates alanine metabolism in muscle cells

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Author
    Hatazawa, Y.
    Qian, K.
    Gong, D.-W.
    Date
    2018
    Journal
    PLoS ONE
    Publisher
    Public Library of Science
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://dx.doi.org/10.1371/journal.pone.0190904
    Abstract
    he skeletal muscle is the largest organ in the human body, depositing energy as protein/amino acids, which are degraded in catabolic conditions such as fasting. Alanine is synthesized and secreted from the skeletal muscle that is used as substrates of gluconeogenesis in the liver. During fasting, the expression of PGC-1α, a transcriptional coactivator of nuclear receptors, is increased in the liver and regulates gluconeogenesis. In the present study, we observed increased mRNA expression of PGC-1α and alanine aminotransferase 2 (ALT2) in the skeletal muscle during fasting. In C2C12 myoblast cells overexpressing PGC-1α, ALT2 expression was increased concomitant with an increased alanine level in the cells and medium. In addition, PGC-1α, along with nuclear receptor ERR, dose-dependently enhanced the ALT2 promoter activity in reporter assay using C2C12 cells. In the absence of glucose in the culture medium, mRNA levels of PGC-1α and ALT2 increased. Endogenous PGC-1α knockdown in C2C12 cells reduced ALT2 gene expression level, induced by the no-glucose medium. Taken together, in the skeletal muscle, PGC-1α activates ALT2 gene expression, and alanine production may play roles in adaptation to fasting.
    Keyword
    Alanine
    Alanine Transaminase
    Animals
    Cell LineFasting
    Gene Expression Regulation
    Liver
    MiceMice, Inbred C57BL
    Muscle, Skeletal
    Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
    Promoter Regions, Genetic
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040245015&doi=10.1371%2fjournal.pone.0190904&partnerID=40&md5=dd614656d29c36ba08d463e0b256ad7c; http://hdl.handle.net/10713/9039
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0190904
    Scopus Count
    Collections
    UMB Open Access Articles

    entitlement

    Related articles

    • Isoform-specific increases in murine skeletal muscle peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) mRNA in response to beta2-adrenergic receptor activation and exercise.
    • Authors: Miura S, Kai Y, Kamei Y, Ezaki O
    • Issue date: 2008 Sep
    • An increase in murine skeletal muscle peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) mRNA in response to exercise is mediated by beta-adrenergic receptor activation.
    • Authors: Miura S, Kawanaka K, Kai Y, Tamura M, Goto M, Shiuchi T, Minokoshi Y, Ezaki O
    • Issue date: 2007 Jul
    • Regulation of PGC-1α expression by a GSK-3β-TFEB signaling axis in skeletal muscle.
    • Authors: Theeuwes WF, Gosker HR, Schols AMWJ, Langen RCJ, Remels AHV
    • Issue date: 2020 Feb
    • Metabolomic Analysis of the Skeletal Muscle of Mice Overexpressing PGC-1α.
    • Authors: Hatazawa Y, Senoo N, Tadaishi M, Ogawa Y, Ezaki O, Kamei Y, Miura S
    • Issue date: 2015
    • Whole body overexpression of PGC-1alpha has opposite effects on hepatic and muscle insulin sensitivity.
    • Authors: Liang H, Balas B, Tantiwong P, Dube J, Goodpaster BH, O'Doherty RM, DeFronzo RA, Richardson A, Musi N, Ward WF
    • Issue date: 2009 Apr
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.