• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Conjugative transposons and their cargo genes vary across natural populations of Rickettsia buchneri infecting the tick Ixodes scapularis

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Author
    Hagen, R.
    Verhoeve, V.I.
    Gillespie, J.J.
    Date
    2018
    Journal
    Genome Biology and Evolution
    Publisher
    Oxford University Press
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://dx.doi.org/10.1093/gbe/evy247
    Abstract
    Rickettsia buchneri (formerly Rickettsia endosymbiont of Ixodes scapularis, or REIS) is an obligate intracellular endoparasite of the black-legged tick, the primary vector of Lyme disease in North America. It is noteworthy among the rickettsiae for its relatively large genome (1.8 Mb) and extraordinary proliferation of mobile genetic elements (MGEs), which comprise nearly 35% of its genome. Previous analysis of the R. buchneri genome identified several integrative conjugative elements named Rickettsiales amplified genomic elements (RAGEs); the composition of these RAGEs suggests that continued genomic invasions by MGEs facilitated the proliferation of rickettsial genes related to an intracellular lifestyle. In this study, we compare the genomic diversity at RAGE loci among sequenced rickettsiae that infect three related Ixodes spp., including two strains of R. buchneri and Rickettsia endosymbiont of Ixodes pacificus strain Humboldt, as well as a closely related species R. tamurae infecting Amblyomma testudinarium ticks. We further develop a novel multiplex droplet digital PCR assay and use it to quantify copy number ratios of chromosomal R. buchneri RAGE-A and RAGE-B to the single-copy gene gltA within natural populations of I. scapularis. Our results reveal substantial diversity among R. buchneri at these loci, both within individual ticks as well as in the I. scapularis population at large, demonstrating that genomic rearrangement of MGEs is an active process in these intracellular bacteria. Copyright The Author(s) 2018.
    Sponsors
    We would like to thank the West Virginia State Department of Epidemiology (Zoonotic Disease Division) for providing the ticks for this study, and Kevin Macaluso (Louisiana State University School of Veterinary Medicine) for providing R. amblyommatis DNA used as a ddPCR control. We are also grateful to Lucy Weinert (University of Cambridge) for sharing the unpublished genomes of Rickettsia species isolated from the ladybird beetle A. bipunctata and the parasitic ciliate I. multifiliis. This work was supported by West Virginia University start-up funds to T.P.D. J.J.G. acknowledges support from the National Institute of Health/National Institute of Allergy and Infectious Diseases grants R01AI017828, R01AI126853, and R21AI26108. The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the article.
    Keyword
    Black-legged tick
    Droplet digital PCR
    Integrative conjugative element
    RAGE
    REIS
    Spotted fever group rickettsiae
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058902876&doi=10.1093%2fgbe%2fevy247&partnerID=40&md5=7a63520bf358dc9ab727808909236f99; http://hdl.handle.net/10713/9030
    ae974a485f413a2113503eed53cd6c53
    10.1093/gbe/evy247
    Scopus Count
    Collections
    UMB Open Access Articles

    entitlement

    Related articles

    • A Rickettsia genome overrun by mobile genetic elements provides insight into the acquisition of genes characteristic of an obligate intracellular lifestyle.
    • Authors: Gillespie JJ, Joardar V, Williams KP, Driscoll T, Hostetler JB, Nordberg E, Shukla M, Walenz B, Hill CA, Nene VM, Azad AF, Sobral BW, Caler E
    • Issue date: 2012 Jan
    • Cryptic Genes for Interbacterial Antagonism Distinguish Rickettsia Species Infecting Blacklegged Ticks From Other Rickettsia Pathogens.
    • Authors: Verhoeve VI, Fauntleroy TD, Risteen RG, Driscoll TP, Gillespie JJ
    • Issue date: 2022
    • The Ixodes scapularis Symbiont Rickettsia buchneri Inhibits Growth of Pathogenic Rickettsiaceae in Tick Cells: Implications for Vector Competence.
    • Authors: Cull B, Burkhardt NY, Wang XR, Thorpe CJ, Oliver JD, Kurtti TJ, Munderloh UG
    • Issue date: 2021
    • Growth Dynamics and Antibiotic Elimination of Symbiotic Rickettsia buchneri in the Tick Ixodes scapularis (Acari: Ixodidae).
    • Authors: Oliver JD, Price LD, Burkhardt NY, Heu CC, Khoo BS, Thorpe CJ, Kurtti TJ, Munderloh UG
    • Issue date: 2021 Jan 15
    • Molecular detection of rickettsial bacteria in ticks of the genus Ixodes from the Southern Cone of America.
    • Authors: Sebastian PS, Flores FS, Saracho-Bottero MN, Tarragona EL, Venzal JM, Nava S
    • Issue date: 2020 Oct
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.