• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles 2018
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles 2018
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Defective mutations within the translocation domain of Clostridium difficile toxin B impair disease pathogenesis

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Author
    Hamza, T.
    Zhang, Z.
    Melnyk, R.A.
    Date
    2018
    Journal
    Pathogens and Disease
    Publisher
    Oxford University Press
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://dx.doi.org/10.1093/femspd/ftv098
    Abstract
    The Clostridium difficile toxin B is one of the main virulence factors and plays an important role in the pathogenesis of C. difficile infection (CDI). We recently revealed crucial residues in the translocation domain of TcdB for the pore formation and toxin translocation. In this study, we investigated the effects of mutating a critical site involved in pore formation, Leu-1106, to residues that differ in size and polarity (Phe, Ala, Cys, Asp). We observed a broad range of effects on TcdB function in vitro consistent with the role of this site in pore formation and translocation. We show that mice challenged systemically with a lethal dose (LD100) of the most defective mutant (L1106K) showed no symptoms of disease highlighting the importance of this residue and the translocation domain in disease pathogenesis. These findings offer insights into the structure function of the toxin translocation pore, and inform novel therapeutic strategies against CDI. Copyright FEMS 2015.
    Keyword
    Clostridium difficile
    TcdB
    Toxin
    Translocation domain
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-84965051355&doi=10.1093%2ffemspd%2fftv098&partnerID=40&md5=046470dafeeb36908eb26a1d0b48bb73; http://hdl.handle.net/10713/8960
    ae974a485f413a2113503eed53cd6c53
    10.1093/femspd/ftv098
    Scopus Count
    Collections
    UMB Open Access Articles 2018

    entitlement

    Related articles

    • Identification of an Essential Region for Translocation of Clostridium difficile Toxin B.
    • Authors: Chen S, Wang H, Gu H, Sun C, Li S, Feng H, Wang J
    • Issue date: 2016 Aug 15
    • Defining the Roles of TcdA and TcdB in Localized Gastrointestinal Disease, Systemic Organ Damage, and the Host Response during Clostridium difficile Infections.
    • Authors: Carter GP, Chakravorty A, Pham Nguyen TA, Mileto S, Schreiber F, Li L, Howarth P, Clare S, Cunningham B, Sambol SP, Cheknis A, Figueroa I, Johnson S, Gerding D, Rood JI, Dougan G, Lawley TD, Lyras D
    • Issue date: 2015 Jun 2
    • Translocation domain mutations affecting cellular toxicity identify the Clostridium difficile toxin B pore.
    • Authors: Zhang Z, Park M, Tam J, Auger A, Beilhartz GL, Lacy DB, Melnyk RA
    • Issue date: 2014 Mar 11
    • Structural determinants for membrane insertion, pore formation and translocation of Clostridium difficile toxin B.
    • Authors: Genisyuerek S, Papatheodorou P, Guttenberg G, Schubert R, Benz R, Aktories K
    • Issue date: 2011 Mar
    • Importance of toxin A, toxin B, and CDT in virulence of an epidemic Clostridium difficile strain.
    • Authors: Kuehne SA, Collery MM, Kelly ML, Cartman ST, Cockayne A, Minton NP
    • Issue date: 2014 Jan 1
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.