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    Effects of PTEN loss and activated KRAS overexpression on mechanical properties of breast epithelial cells

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    Author
    Linthicum, W.
    Thanh, M.-T.H.
    Vitolo, M.I.
    Date
    2018
    Journal
    International Journal of Molecular Sciences
    Publisher
    MDPI AG
    Type
    Article
    
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    See at
    https://dx.doi.org/10.3390/ijms19061613
    Abstract
    It has previously been shown that the simultaneous activation of PI3K (phosphatidylinositol 3-kinase) and Ras/MAPK (mitogen-activated protein kinases) pathways facilitate tumor growth despite only inducing cancer cell dormancy individually. Determining the impacts on cellular mechanics each pathway incites alone and in unison is critical to developing non-toxic cancer therapies for triple-negative breast cancers. PTEN (phosphatase and tensin homolog) knockout and activated KRAS (Kristen rat sarcoma viral oncogene homolog) overexpression in healthy MCF-10A human breast epithelial cells activated the PI3K and Ras/MAPK pathways, respectively. Cell stiffness and fluidity were simultaneously measured using atomic force microscopy. Results suggest that PTEN knockout reduced cell stiffness and increased cell fluidity independent of PI3K activation. Effects of activated KRAS overexpression on cell stiffness depends on rigidity of cell culture substrate. Activated KRAS overexpression also counteracts the effects of PTEN knockout. Copyright 2018 by the authors. Licensee MDPI, Basel, Switzerland.
    Sponsors
    Acknowledgments: This research is supported by NSF grant CBET-1403257 (Q.W.), IGERT-DGE-1144804 (W.L.), NIH grant K01-CA166576 (M.I.V.), and 122229-IRG-97-153-10-IRG from the American Cancer Society (M.I.V.).
    Keyword
    Atomic force microscopy
    Cancer
    Cell mechanics
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047907998&doi=10.3390%2fijms19061613&partnerID=40&md5=95ad1e5b9a644e9b01558d5140d006c5; http://hdl.handle.net/10713/8938
    ae974a485f413a2113503eed53cd6c53
    10.3390/ijms19061613
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    UMB Open Access Articles 2018

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