• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles 2019
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles 2019
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Donor-derived cell-free DNA predicts allograft failure and mortality after lung transplantation

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Publisher version
    View Source
    Access full-text PDFOpen Access
    View Source
    Check access options
    Check access options
    Author
    Agbor-Enoh, S.
    Wang, Y.
    Tunc, I.
    Date
    2019
    Journal
    EBioMedicine
    Publisher
    Elsevier B.V.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://dx.doi.org/10.1016/j.ebiom.2018.12.029
    Abstract
    Background: Allograft failure is common in lung-transplant recipients and leads to poor outcomes including early death. No reliable clinical tools exist to identify patients at high risk for allograft failure. This study tested the use of donor-derived cell-free DNA (%ddcfDNA) as a sensitive marker of early graft injury to predict impending allograft failure. Methods: This multicenter, prospective cohort study enrolled 106 subjects who underwent lung transplantation and monitored them after transplantation for the development of allograft failure (defined as severe chronic lung allograft dysfunction [CLAD], retransplantation, and/or death from respiratory failure). Plasma samples were collected serially in the first three months following transplantation and assayed for %ddcfDNA by shotgun sequencing. We computed the average levels of ddcfDNA over three months for each patient (avddDNA) and determined its relationship to allograft failure using Cox-regression analysis. Findings: avddDNA was highly variable among subjects: median values were 3.6%, 1.6% and 0.7% for the upper, middle, and low tertiles, respectively (range 0.1%-9.9%). Compared to subjects in the low and middle tertiles, those with avddDNA in the upper tertile had a 6.6-fold higher risk of developing allograft failure (95% confidence interval 1.6-19.9, p = 0.007), lower peak FEV1 values, and more frequent %ddcfDNA elevations that were not clinically detectable. Interpretation: Lung transplant patients with early unresolving allograft injury measured via %ddcfDNA are at risk of subsequent allograft injury, which is often clinically silent, and progresses to allograft failure. Fund: National Institutes of Health. © 2019
    Sponsors
    The Genome Transplant Dynamics Study ( NCT01985412 ) is funded by the National Institutes of Health , Grant RC4AI092673 . The Genomic Research Alliance for Transplantation Study ( NCT02423070 ) is funded by the Division of Intramural Research of the National Heart, Lung, and Blood Institute . Funders had no role in study design, data collection, data analysis, interpretation, writing of the report.
    Keyword
    allograft failure
    donor-derived cell-free DNA
    Allografts
    Biomarkers
    Lung Transplantation--mortality
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85060510387&doi=10.1016%2fj.ebiom.2018.12.029&partnerID=40&md5=ade58930f69b7b6f7e2130d51198fb03; http://hdl.handle.net/10713/8725
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.ebiom.2018.12.029
    Scopus Count
    Collections
    UMB Open Access Articles 2019

    entitlement

    Related articles

    • Late manifestation of alloantibody-associated injury and clinical pulmonary antibody-mediated rejection: Evidence from cell-free DNA analysis.
    • Authors: Agbor-Enoh S, Jackson AM, Tunc I, Berry GJ, Cochrane A, Grimm D, Davis A, Shah P, Brown AW, Wang Y, Timofte I, Shah P, Gorham S, Wylie J, Goodwin N, Jang MK, Marishta A, Bhatti K, Fideli U, Yang Y, Luikart H, Cao Z, Pirooznia M, Zhu J, Marboe C, Iacono A, Nathan SD, Orens J, Valantine HA, Khush K
    • Issue date: 2018 Jul
    • Applying rigor and reproducibility standards to assay donor-derived cell-free DNA as a non-invasive method for detection of acute rejection and graft injury after heart transplantation.
    • Authors: Agbor-Enoh S, Tunc I, De Vlaminck I, Fideli U, Davis A, Cuttin K, Bhatti K, Marishta A, Solomon MA, Jackson A, Graninger G, Harper B, Luikart H, Wylie J, Wang X, Berry G, Marboe C, Khush K, Zhu J, Valantine H
    • Issue date: 2017 Sep
    • De Novo DQ Donor-Specific Antibodies Are Associated with Chronic Lung Allograft Dysfunction after Lung Transplantation.
    • Authors: Tikkanen JM, Singer LG, Kim SJ, Li Y, Binnie M, Chaparro C, Chow CW, Martinu T, Azad S, Keshavjee S, Tinckam K
    • Issue date: 2016 Sep 1
    • Erratic tacrolimus exposure, assessed using the standard deviation of trough blood levels, predicts chronic lung allograft dysfunction and survival.
    • Authors: Gallagher HM, Sarwar G, Tse T, Sladden TM, Hii E, Yerkovich ST, Hopkins PM, Chambers DC
    • Issue date: 2015 Nov
    • Both Pre-Transplant and Early Post-Transplant Antireflux Surgery Prevent Development of Early Allograft Injury After Lung Transplantation.
    • Authors: Lo WK, Goldberg HJ, Wee J, Fisichella PM, Chan WW
    • Issue date: 2016 Jan
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.