• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Programmed genome editing of the omega-1 ribonuclease of the blood fluke, Schistosoma mansoni

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Author
    Ittiprasert, W.
    Mann, V.H.
    Karinshak, S.E.
    Date
    2019
    Journal
    eLife
    Publisher
    eLife
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://dx.doi.org/10.7554/eLife.41337
    Abstract
    CRISPR/Cas9-based genome editing has yet to be reported in species of the Platyhelminthes. We tested this approach by targeting omega-1 (ω1) of Schistosoma mansoni as proof of principle. This secreted ribonuclease is crucial for Th2 polarization and granuloma formation. Schistosome eggs were exposed to Cas9 complexed with guide RNA complementary to ω1 by electroporation or by transduction with lentiviral particles. Some eggs were also transfected with a single stranded donor template. Sequences of amplicons from gene-edited parasites exhibited Cas9-catalyzed mutations including homology directed repaired alleles, and other analyses revealed depletion of ω1 transcripts and the ribonuclease. Gene-edited eggs failed to polarize Th2 cytokine responses in macrophage/T-cell co-cultures, while the volume of pulmonary granulomas surrounding ω1-mutated eggs following tail-vein injection into mice was vastly reduced. Knock-out of ω1 and the diminished levels of these cytokines following exposure showcase the novel application of programmed gene editing for functional genomics in schistosomes.
    Sponsors
    National Institute of Allergy and Infectious Diseases (R21AI109532) -Gabriel Rinaldi ; Thailand Research Fund (PHD/0011/2555) - Apisit Chaidee; Thailand Research Fund (PHD/0047/2556) - Pannathee Prangtaworn; Thailand Research Fund (PHD/00531/2556) - Rutchanee Rodpai; Wellcome (107475/Z/15/Z) - Karl F Hoffmann; Wellcome (WT 098051) - Matthew Berriman; MaxMind Inc - Paul J Brindley. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
    Keyword
    CRISPR/Cas9
    granuloma
    infectious disease
    microbiology
    omega-1
    ribonuclease
    Schistosoma mansoni
    Th2-polarization
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85060919508&doi=10.7554%2feLife.41337&partnerID=40&md5=e2dea6590a67e0f640bfdf5f64912d49; http://hdl.handle.net/10713/8718
    ae974a485f413a2113503eed53cd6c53
    10.7554/eLife.41337
    Scopus Count
    Collections
    UMB Open Access Articles

    entitlement

    Related articles

    • CRISPR/Cas9-mediated genome editing of Schistosoma mansoni acetylcholinesterase.
    • Authors: You H, Mayer JU, Johnston RL, Sivakumaran H, Ranasinghe S, Rivera V, Kondrashova O, Koufariotis LT, Du X, Driguez P, French JD, Waddell N, Duke MG, Ittiprasert W, Mann VH, Brindley PJ, Jones MK, McManus DP
    • Issue date: 2021 Jan
    • RNA-Guided AsCas12a- and SpCas9-Catalyzed Knockout and Homology Directed Repair of the Omega-1 Locus of the Human Blood Fluke, Schistosoma mansoni.
    • Authors: Ittiprasert W, Chatupheeraphat C, Mann VH, Li W, Miller A, Ogunbayo T, Tran K, Alrefaei YN, Mentink-Kane M, Brindley PJ
    • Issue date: 2022 Jan 6
    • Molecular characterization of omega-1: a hepatotoxic ribonuclease from Schistosoma mansoni eggs.
    • Authors: Fitzsimmons CM, Schramm G, Jones FM, Chalmers IW, Hoffmann KF, Grevelding CG, Wuhrer M, Hokke CH, Haas H, Doenhoff MJ, Dunne DW
    • Issue date: 2005 Nov
    • Analysis of cytokine mRNA expression during primary granuloma formation induced by eggs of Schistosoma mansoni.
    • Authors: Wynn TA, Eltoum I, Cheever AW, Lewis FA, Gause WC, Sher A
    • Issue date: 1993 Aug 1
    • TALEN- and CRISPR-enhanced DNA homologous recombination for gene editing in zebrafish.
    • Authors: Zhang Y, Huang H, Zhang B, Lin S
    • Issue date: 2016
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.