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dc.contributor.authorRodríguez-Martínez, Alba
dc.contributor.authorde Miguel-Pérez, Diego
dc.contributor.authorOrtega, Francisco Gabriel
dc.date.accessioned2019-03-29T14:47:37Z
dc.date.available2019-03-29T14:47:37Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85061121429&doi=10.1186%2fs13058-019-1109-0&partnerID=40&md5=7f4d87c6e2acf012560d3c82c23afbf8
dc.identifier.urihttp://hdl.handle.net/10713/8692
dc.description.abstractBackground: Breast cancer patients under neoadjuvant chemotherapy includes a heterogeneous group of patients who eventually develop distal disease, not detectable by current methods. We propose the use of exosomal miRNAs and circulating tumor cells as diagnostic and predictive biomarkers in these patients. Methods: Fifty-three breast cancer women initially diagnosed with localized breast cancer under neoadjuvant chemotherapy were prospectively enrolled in this study. However, six of them were later re-evaluated and diagnosed as metastatic breast cancer patients by PET-CT scan. Additionally, eight healthy donors were included. Circulating tumor cells and serum exosomal miRNAs were isolated from blood samples before and at the middle of neoadjuvant therapy and exosomal miRNA levels analyzed by qPCR. Results: Before neoadjuvant therapy, exosomal miRNA-21 and 105 expression levels were higher in metastatic versus non-metastatic patients and healthy donors. Likewise, higher levels of miRNA-222 were observed in basal-like (p = 0.037) and in luminal B versus luminal A (p = 0.0145) tumor subtypes. Exosomal miRNA-222 levels correlated with clinical and pathological variables such as progesterone receptor status (p = 0.017) and Ki67 (p = 0.05). During neoadjuvant treatment, exosomal miRNA-21 expression levels directly correlated with tumor size (p = 0.039) and inversely with Ki67 expression (p = 0.031). Finally, higher levels of exosomal miRNA-21, miRNA-222, and miRNA-155 were significantly associated with the presence of circulating tumor cells. Conclusion: Liquid biopsies based on exosomal miRNAs and circulating tumor cells can be a complementary clinical tool for improving breast cancer diagnosis and prognosis. Copyright 2019 The Author(s).en_US
dc.description.sponsorshipThis work was supported by "Granada Research of Excellence Initiative on BioHealth (GREIB)", the PhD grant from the University of Granada and the PhD grant from the Spanish Government (FPU) 2014, REF FPU14/05461.en_US
dc.description.urihttps://dx.doi.org/10.1186/s13058-019-1109-0en_US
dc.language.isoen_USen_US
dc.publisherBioMed Central Ltd.en_US
dc.relation.ispartofBreast Cancer Research
dc.subjectcirculating tumor cellsen_US
dc.subjectconservative surgeryen_US
dc.subjectlocalized breast canceren_US
dc.subjectneoadjuvant chemotherapyen_US
dc.subject.lcshCancer--Diagnosisen_US
dc.subject.lcshCancer--Prognosisen_US
dc.subject.meshExosomesen_US
dc.subject.meshMicroRNAsen_US
dc.titleExosomal miRNA profile as complementary tool in the diagnostic and prediction of treatment response in localized breast cancer under neoadjuvant chemotherapyen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/s13058-019-1109-0
dc.identifier.pmid30728048


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