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dc.contributor.authorLedwaba, S.E.
dc.contributor.authorBolick, D.T.
dc.contributor.authorMedeiros, P.H.Q.S.en_US
dc.date.accessioned2019-03-29T14:47:36Z
dc.date.available2019-03-29T14:47:36Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85060998027&doi=10.1080%2f19490976.2018.1564430&partnerID=40&md5=02d727781b9ad44cd04bcfa0d7b99ffd
dc.identifier.urihttp://hdl.handle.net/10713/8684
dc.description.abstractShigella is one of the major enteric pathogens worldwide. We present a murine model of S. flexneri infection and investigate the role of zinc deficiency (ZD). C57BL/6 mice fed either standard chow (HC) or ZD diets were pretreated with an antibiotic cocktail and received S. flexneri strain 2457T orally. Antibiotic pre-treated ZD mice showed higher S. flexneri colonization than non-treated mice. ZD mice showed persistent colonization for at least 50 days post-infection (pi). S. flexneri-infected mice showed significant weight loss, diarrhea and increased levels of fecal MPO and LCN in both HC and ZD fed mice. S. flexneri preferentially colonized the colon, caused epithelial disruption and inflammatory cell infiltrate, and promoted cytokine production which correlated with weight loss and histopathological changes. Infection with S. flexneri ΔmxiG (critical for type 3 secretion system) did not cause weight loss or diarrhea, and had decreased stool shedding duration and tissue burden. Several biochemical changes related to energy, inflammation and gut-microbial metabolism were observed. Zinc supplementation increased weight gains and reduced intestinal inflammation and stool shedding in ZD infected mice. In conclusion, young antibiotic-treated mice provide a new model of oral S. flexneri infection, with ZD promoting prolonged infection outcomes. Copyright 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.en_US
dc.description.urihttps://dx.doi.org/10.1080/19490976.2018.1564430en_US
dc.language.isoen_USen_US
dc.publisherTaylor and Francis Inc.en_US
dc.relation.ispartofGut Microbes
dc.subjectintestinal microbiotaen_US
dc.subjectmouse modelen_US
dc.subjectShigellosisen_US
dc.subjecturine metabolomicsen_US
dc.subjectzinc deficiencyen_US
dc.titleA murine model of diarrhea, growth impairment and metabolic disturbances with Shigella flexneri infection and the role of zinc deficiencyen_US
dc.typeArticleen_US
dc.identifier.doi10.1080/19490976.2018.1564430


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