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    A murine model of diarrhea, growth impairment and metabolic disturbances with Shigella flexneri infection and the role of zinc deficiency

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    Author
    Ledwaba, S.E.
    Bolick, D.T.
    Medeiros, P.H.Q.S.
    Date
    2019
    Journal
    Gut Microbes
    Publisher
    Taylor and Francis Inc.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://dx.doi.org/10.1080/19490976.2018.1564430
    Abstract
    Shigella is one of the major enteric pathogens worldwide. We present a murine model of S. flexneri infection and investigate the role of zinc deficiency (ZD). C57BL/6 mice fed either standard chow (HC) or ZD diets were pretreated with an antibiotic cocktail and received S. flexneri strain 2457T orally. Antibiotic pre-treated ZD mice showed higher S. flexneri colonization than non-treated mice. ZD mice showed persistent colonization for at least 50 days post-infection (pi). S. flexneri-infected mice showed significant weight loss, diarrhea and increased levels of fecal MPO and LCN in both HC and ZD fed mice. S. flexneri preferentially colonized the colon, caused epithelial disruption and inflammatory cell infiltrate, and promoted cytokine production which correlated with weight loss and histopathological changes. Infection with S. flexneri ΔmxiG (critical for type 3 secretion system) did not cause weight loss or diarrhea, and had decreased stool shedding duration and tissue burden. Several biochemical changes related to energy, inflammation and gut-microbial metabolism were observed. Zinc supplementation increased weight gains and reduced intestinal inflammation and stool shedding in ZD infected mice. In conclusion, young antibiotic-treated mice provide a new model of oral S. flexneri infection, with ZD promoting prolonged infection outcomes. Copyright 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.
    Keyword
    intestinal microbiota
    mouse model
    Shigellosis
    urine metabolomics
    zinc deficiency
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85060998027&doi=10.1080%2f19490976.2018.1564430&partnerID=40&md5=02d727781b9ad44cd04bcfa0d7b99ffd; http://hdl.handle.net/10713/8684
    ae974a485f413a2113503eed53cd6c53
    10.1080/19490976.2018.1564430
    Scopus Count
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    UMB Open Access Articles 2019

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