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dc.contributor.authorZhou, Y.
dc.contributor.authorWang, S.
dc.contributor.authorZhou, X.
dc.date.accessioned2019-03-29T14:47:36Z
dc.date.available2019-03-29T14:47:36Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85061330343&doi=10.1155%2f2019%2f6393470&partnerID=40&md5=4eed21b690574bf949d6c013b8977dbd
dc.identifier.urihttp://hdl.handle.net/10713/8682
dc.description.abstractQuaternary ammonium compounds constitute a large group of antibacterial chemicals with a potential for inhibiting dental plaque. The aims of this study were to evaluate short-time antibacterial and regulating effects of dimethylaminododecyl methacrylate (DMADDM) on multispecies biofilm viability, reformation, and bacterial composition in vitro. DMADDM, chlorhexidine (CHX), and sodium fluoride (NaF) were chosen in the present study. Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii were used to form multispecies biofilm. Cytotoxicity assay was used to determine the optimal tested concentration. 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and resazurin test of biofilm were conducted to study the biomass changes and metabolic changes of controlled multispecies biofilm. Scanning electron microscopy (SEM) was used to observe biofilm images. TaqMan real-time polymerase chain reaction was performed to evaluate the proportion change in multispecies biofilm of different groups. Cytotoxicity assay showed that there existed a certain concentration application range for DMADDM, CHX, and NaF. MTT assay and resazurin test results showed that DMADDM and CHX groups decreased multispecies biofilm growth and metabolic activity (p < 0.05), no matter after 1 min or 5 min direct contact killing or after 24 h regrowth. The proportion of S. mutans decreased steadily in DMADDM and CHX groups after 1 min and 5 min direct contact killing and 24 h regrowth, compared to control groups. A novel DMADDM-containing solution was developed, achieving effective short-time antibacterial effects and regulation ability of biofilm formation. Copyright 2019 Yujie Zhou et al.en_US
dc.description.sponsorshipThis study was supported by the National Key Research Program of China 2017YFC0840100 (Lei Cheng) and 2017YFC0840107 (Lei Cheng), National Natural Science Foundation of China 81430011 (Xuedong Zhou), the Youth Grant of the Science and Technology Department of Sichuan Province, China 2017JQ0028 (Lei Cheng), Innovative Research Team Program of Sichuan Province (Lei Cheng), NIH R01 DE17974 (Hockin H. K. Xu), University of Maryland School of Dentistry bridge fund (Hockin H. K. Xu), and Special Key Laboratory of Oral Disease Research of Higher Education Institution Program of Guizhou Province SKLODR201701 (Qi Han).en_US
dc.description.urihttps://dx.doi.org/10.1155/2019/6393470en_US
dc.language.isoen_USen_US
dc.publisherHindawi Limiteden_US
dc.relation.ispartofBioMed Research International
dc.subjectdimethylaminododecyl methacrylateen_US
dc.subject.meshBiofilmsen_US
dc.subject.meshAnti-Bacterial Agentsen_US
dc.subject.meshOral Healthen_US
dc.titleShort-time antibacterial effects of dimethylaminododecyl methacrylate on oral multispecies biofilm in vitroen_US
dc.typeArticleen_US
dc.identifier.doi10.1155/2019/6393470
dc.identifier.pmid30800673


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