Biofilm Formation of Staphylococcus aureus under Food Heat Processing Conditions: First Report on CML Production within Biofilm
Date
2019Journal
Scientific ReportsPublisher
Nature Publishing GroupType
Article
Metadata
Show full item recordAbstract
This study aimed to evaluate the Staphylococcus aureus biofilm formation and Nε-carboxymethyl-lysine generation ability under food heat processing conditions including pH (5.0-9.0), temperature (25°C, 31°C, 37°C, 42°C and 65°C), NaCl concentration (10%, 15% and 20%, w/v) and glucose concentration (0.5%, 1%, 2%, 3%, 5%, 10%, w/v). S. aureus biofilm genetic character was obtained by PCR detecting atl, ica operon, sasG and agr. Biofilm biomass and metabolic activity were quantified with crystal violet and methyl thiazolyl tetrazolium staining methods. S. aureus biofilm was sensitive to food heat processing conditions with 37°C, pH 7.0, 2% glucose concentration (w/v) and 10% NaCl concentration (w/v) were favorable conditions. Besides, free and bound Nε-carboxymethyl-lysine level in weak, moderate and strong biofilm were detected by optimized high performance liquid chromatography tandem mass spectrometry. Nε-carboxymethyl-lysine level in S. aureus biofilm possessed a significant gap between strong, moderate and weak biofilm strains. This investigation revealed the biological and chemical hazard of Staphylococcus aureus biofilm to food processing environment. Copyright 2019, The Author(s).Keyword
Nε-carboxymethyl-lysineStaphylococcus aureus
Biofilms
Food Handling
Hot Temperature--adverse effects
Identifier to cite or link to this item
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85061039172&doi=10.1038%2fs41598-018-35558-2&partnerID=40&md5=1fd974191ef71bef5e90a2340f1a8a23; http://hdl.handle.net/10713/8670ae974a485f413a2113503eed53cd6c53
10.1038/s41598-018-35558-2
Scopus Count
Collections
Related articles
- Impact of food-related environmental factors on the adherence and biofilm formation of natural Staphylococcus aureus isolates.
- Authors: Vázquez-Sánchez D, Habimana O, Holck A
- Issue date: 2013 Feb
- Diverse Profiles of Biofilm and Adhesion Genes in Staphylococcus Aureus Food Strains Isolated from Sushi and Sashimi.
- Authors: Puah SM, Tan JAMA, Chew CH, Chua KH
- Issue date: 2018 Sep
- Different patterns of biofilm formation in Staphylococcus aureus under food-related stress conditions.
- Authors: Rode TM, Langsrud S, Holck A, Møretrø T
- Issue date: 2007 May 30
- Modeling the effect of pH, water activity, and ethanol concentration on biofilm formation of Staphylococcus aureus.
- Authors: Tango CN, Akkermans S, Hussain MS, Khan I, Van Impe J, Jin YG, Oh DH
- Issue date: 2018 Dec
- Crystal Violet and XTT Assays on Staphylococcus aureus Biofilm Quantification.
- Authors: Xu Z, Liang Y, Lin S, Chen D, Li B, Li L, Deng Y
- Issue date: 2016 Oct