A novel model for studying voltage-gated ion channel gene expression during reversible ischemic stroke
JournalInternational Journal of Medical Sciences
PublisherIvyspring International Publisher
MetadataShow full item record
AbstractThe dysfunction of voltage-gated ion channels contributes to the pathology of ischemic stroke. In this study, we developed rat models of transient ischemic attack (TIA) and reversible ischemic neurological deficit (RIND) that was induced via the injection of artificial embolic particles during full consciousness, that allow us to monitor the neurologic deficit and positron emission tomography (PET) scans in real-time. We then evaluated the infarction volume of brain tissue was confirmed by 2,3,5-triphenyl tetrazolium chloride (TTC) staining, and gene expressions were evaluated by quantitative real-time PCR (qPCR). We found that rats with TIA or RIND exhibited neurological deficits as determined by negative TTC and PET findings. However, the expression of voltage-gated sodium channels in the hippocampus was significantly up-regulated in the qPCR array study. Furthermore, an altered expression of sodium channel ?-subunits and potassium channels, were observed in RIND compared to TIA groups. In conclusion, to our knowledge, this is the first report of the successful evaluation of voltage-gated ion channel gene expression in TIA and RIND animal models. This model will aid future studies in investigating pathophysiological mechanisms, and in developing new therapeutic compounds for the treatment of TIA and RIND. © Ivyspring International Publisher.
SponsorsThe authors thank the support provided by the MOST (106-2320-B-650-002-), KMUDK106, NCKUED A10612, and NSYSU-KMU Joint Research Project (KMU-NSYSU 107-I001).
Reversible ischemic neurological deficit
Transient ischemic attack
Voltage-gated ion channels
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85060149849&doi=10.7150%2fijms.27442&partnerID=40&md5=972939eb20b9b9e0c989ac010e25c367; http://hdl.handle.net/10713/8639
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