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dc.contributor.authorBooth, J.S.
dc.contributor.authorGoldberg, E.
dc.contributor.authorPatil, S.A.
dc.date.accessioned2019-03-29T14:42:02Z
dc.date.available2019-03-29T14:42:02Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85061573766&doi=10.1093%2fintimm%2fdxy070&partnerID=40&md5=d1a7456585d2f6fdae894843bffbcc05
dc.identifier.urihttp://hdl.handle.net/10713/8598
dc.description.abstractOur current understanding of CD4 + T-cell-mediated immunity (CMI) elicited by the oral live attenuated typhoid vaccine Ty21a is primarily derived from studies using peripheral blood. Very limited data are available in humans regarding mucosal immunity (especially CD4 + T) at the site of infection (e.g. terminal ileum; TI). Here using multiparametric flow cytometry, we examined the effect of Ty21a immunization on TI-lamina propria mononuclear cells (LPMC) and peripheral blood CD4 + T memory (T M) subsets in volunteers undergoing routine colonoscopy. Interestingly, we observed significant increases in the frequencies of LPMC CD4 + T cells following Ty21a immunization, restricted to the T effector/memory (T EM)-CD45RA + (T EMRA) subset. Importantly, Ty21a immunization elicited Salmonella Typhi-responsive LPMC CD4 + T cells in all major T M subsets [interferon (IFN)γ and interleukin (IL)-17A in T EM; (IFN)γ and macrophage inflammatory protein (MIP)1β in T central/memory (T CM); and IL-2 in T EMRA ]. Subsequently, we analyzed LPMC S. Typhi-responsive CD4 + T cells in depth for multifunctional (MF) effectors. We found that LPMC CD4 + T EM responses were mostly MF, except for those cells exhibiting the characteristics associated with IL-17A responses. Finally, we compared mucosal to systemic responses and observed that LPMC CD4 + S. Typhi-specific responses were unique and distinct from their systemic counterparts. This study provides the first demonstration of S. Typhi-specific CD4 + T M responses in the human TI mucosa and provides valuable information about the generation of mucosal immune responses following oral Ty21a immunization. Copyright The Author(s) 2018. Published by Oxford University Press on behalf of The Japanese Society for Immunology.en_US
dc.description.sponsorshipThis work was supported by NIAID, NIH, DHHS grants R01-AI036525, U19-AI082655 [Cooperative Center for Human Immunology (CCHI)] and U19-AI109776 [Center of Excellence for Translational Research (CETR)]. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Allergy and Infectious Diseases or the National Institutes of Health.en_US
dc.description.urihttps://dx.doi.org/10.1093/intimm/dxy070en_US
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofInternational Immunology
dc.subjectCD4 + Ten_US
dc.subjectlamina propria mononuclear cellsen_US
dc.subjectmucosal responsesen_US
dc.subject.meshBlooden_US
dc.subject.meshTyphoid Feveren_US
dc.titleEffect of the live oral attenuated typhoid vaccine, Ty21a, on systemic and terminal ileum mucosal CD4 + T memory responses in humansen_US
dc.typeArticleen_US
dc.identifier.doi10.1093/intimm/dxy070
dc.identifier.pmid30346608


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