• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    Plasmodium vivax transcriptomes reveal stage-specific chloroquine response and differential regulation of male and female gametocytes

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Author
    Kim, A.
    Popovici, J.
    Menard, D.
    Date
    2019
    Journal
    Nature Communications
    Publisher
    Springer Nature Publishing
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://dx.doi.org/10.1038/s41467-019-08312-z
    Abstract
    Studies of Plasmodium vivax gene expression are complicated by the lack of in vitro culture system and the difficulties associated with studying clinical infections that often contain multiple clones and a mixture of parasite stages. Here, we characterize the transcriptomes of P. vivax parasites from 26 malaria patients. We show that most parasite mRNAs derive from trophozoites and that the asynchronicity of P. vivax infections is therefore unlikely to confound gene expression studies. Analyses of gametocyte genes reveal two distinct clusters of co-regulated genes, suggesting that male and female gametocytes are independently regulated. Finally, we analyze gene expression changes induced by chloroquine and show that this antimalarial drug efficiently eliminates most P. vivax parasite stages but, in contrast to P. falciparum, does not affect trophozoites. © 2019, The Author(s).
    Sponsors
    The authors would like to thank all patients and healthcare workers involved in this study and the staff of the Malaria Molecular Epidemiology Unit at the Institut Pasteur in Cambodia and of the National Center for Parasitology, Entomology and Malaria Control in Cambodia for their collaboration and sample collection. The authors would also like to thank Dr. Kafsack and Dr. Lawniczak for facilitating access to their scRNA-seq data. This work was funded by a National Institutes of Health-NIAID award to D.S. (R01 AI103228).
    Keyword
    Chloroquine
    Plasmodium vivax--genetics
    Plasmodium vivax--growth & development
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85060402444&doi=10.1038%2fs41467-019-08312-z&partnerID=40&md5=8661e1b0a3187139a7a73d01154ddc3c; http://hdl.handle.net/10713/8557
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41467-019-08312-z
    Scopus Count
    Collections
    UMB Open Access Articles

    entitlement

    Related articles

    • Plasmodium vivax chloroquine resistance links to pvcrt transcription in a genetic cross.
    • Authors: Sá JM, Kaslow SR, Moraes Barros RR, Brazeau NF, Parobek CM, Tao D, Salzman RE, Gibson TJ, Velmurugan S, Krause MA, Melendez-Muniz V, Kite WA, Han PK, Eastman RT, Kim A, Kessler EG, Abebe Y, James ER, Chakravarty S, Orr-Gonzalez S, Lambert LE, Engels T, Thomas ML, Fasinu PS, Serre D, Gwadz RW, Walker L, DeConti DK, Mu J, Bailey JA, Sim BKL, Hoffman SL, Fay MP, Dinglasan RR, Juliano JJ, Wellems TE
    • Issue date: 2019 Sep 20
    • Susceptibility of Plasmodium falciparum to artemisinins and Plasmodium vivax to chloroquine in Phuoc Chien Commune, Ninh Thuan Province, south-central Vietnam.
    • Authors: Phong NC, Chavchich M, Quang HH, San NN, Birrell GW, Chuang I, Martin NJ, Manh ND, Edstein MD
    • Issue date: 2019 Jan 17
    • Transmission dynamics of co-endemic Plasmodium vivax and P. falciparum in Ethiopia and prevalence of antimalarial resistant genotypes.
    • Authors: Lo E, Hemming-Schroeder E, Yewhalaw D, Nguyen J, Kebede E, Zemene E, Getachew S, Tushune K, Zhong D, Zhou G, Petros B, Yan G
    • Issue date: 2017 Jul
    • Proteomic approaches to studying drug targets and resistance in Plasmodium.
    • Authors: Cooper RA, Carucci DJ
    • Issue date: 2004 Mar
    • Plasmodium vivax trophozoites insensitive to chloroquine.
    • Authors: Sharrock WW, Suwanarusk R, Lek-Uthai U, Edstein MD, Kosaisavee V, Travers T, Jaidee A, Sriprawat K, Price RN, Nosten F, Russell B
    • Issue date: 2008 May 27
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.