Plasmodium vivax transcriptomes reveal stage-specific chloroquine response and differential regulation of male and female gametocytes
PublisherSpringer Nature Publishing
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AbstractStudies of Plasmodium vivax gene expression are complicated by the lack of in vitro culture system and the difficulties associated with studying clinical infections that often contain multiple clones and a mixture of parasite stages. Here, we characterize the transcriptomes of P. vivax parasites from 26 malaria patients. We show that most parasite mRNAs derive from trophozoites and that the asynchronicity of P. vivax infections is therefore unlikely to confound gene expression studies. Analyses of gametocyte genes reveal two distinct clusters of co-regulated genes, suggesting that male and female gametocytes are independently regulated. Finally, we analyze gene expression changes induced by chloroquine and show that this antimalarial drug efficiently eliminates most P. vivax parasite stages but, in contrast to P. falciparum, does not affect trophozoites. © 2019, The Author(s).
SponsorsThe authors would like to thank all patients and healthcare workers involved in this study and the staff of the Malaria Molecular Epidemiology Unit at the Institut Pasteur in Cambodia and of the National Center for Parasitology, Entomology and Malaria Control in Cambodia for their collaboration and sample collection. The authors would also like to thank Dr. Kafsack and Dr. Lawniczak for facilitating access to their scRNA-seq data. This work was funded by a National Institutes of Health-NIAID award to D.S. (R01 AI103228).
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85060402444&doi=10.1038%2fs41467-019-08312-z&partnerID=40&md5=8661e1b0a3187139a7a73d01154ddc3c; http://hdl.handle.net/10713/8557