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dc.contributor.authorSalerno-Goncalves, Rosangela
dc.contributor.authorTettelin, Herve
dc.contributor.authorLou, David
dc.contributor.authorSteiner, Stephanie
dc.contributor.authorRezwanul, Tasmia
dc.contributor.authorGuo, Qin
dc.contributor.authorPicking, William D.
dc.contributor.authorNene, Vishvanath
dc.contributor.authorSztein, Marcelo B.
dc.date.accessioned2017-10-25T16:14:42Z
dc.date.available2017-10-25T16:14:42Z
dc.date.issued2017-09-05
dc.identifier.citationSalerno-Gonçalves R, Tettelin H, Lou D, et al. (2017). Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells. PLoS Neglected Tropical Diseases, 11(9), e0005912, DOI: https://doi.org/10.1371/journal.pntd.0005912en_US
dc.identifier.urihttp://hdl.handle.net/10713/7234
dc.descriptionResearchers from the University of Maryland School of Medicine, the University of Kansas and the International Livestock Research Institute report their findingsen_US
dc.description.abstractSalmonella enterica serovar Typhi (S. Typhi), the causative agent of the typhoid fever, is a pathogen of great public health importance. Typhoid vaccines have the potential to be cost-effective measures towards combating this disease, yet the antigens triggering host protective immune responses are largely unknown. Given the key role of cellular-mediated immunity in S. Typhi protection, it is crucial to identify S. Typhi proteins involved in T-cell responses. Here, cells from individuals immunized with Ty21a typhoid vaccine were collected before and after immunization and used as effectors. We also used an innovative antigen expressing system based on the infection of B-cells with recombinant Escherichia coli (E. coli) expressing one of four S. Typhi gene products (i.e., SifA, OmpC, FliC, GroEL) as targets. Using flow cytometry, we found that the pattern of response to specific S. Typhi proteins was variable. Some individuals responded to all four proteins while others responded to only one or two proteins. We next evaluated whether T-cells responding to recombinant E. coli also possess the ability to respond to purified proteins. We observed that CD4+ cell responses, but not CD8+ cell responses, to recombinant E. coli were significantly associated with the responses to purified proteins. Thus, our results demonstrate the feasibility of using an E. coli expressing system to uncover the antigen specificity of T-cells and highlight its applicability to vaccine studies. These results also emphasize the importance of selecting the stimuli appropriately when evaluating CD4+ and CD8+ cell responses.en_US
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Department of Health and Human Services (DHHS) federal research grants (https://www.niaid.nih.gov) R01 AI036525, U19 AI082655 (Cooperative Center for Human Immunology [CCHI]) and U19-AI109776 (Center of Excellence for Translational Research [CETR] to MS).en_US
dc.language.isoen_USen_US
dc.publisherSan Francisco: PLOSen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.meshAntigens, Bacterialen_US
dc.subject.meshT-Lymphocytesen_US
dc.subject.meshTyphoid-Paratyphoid Vaccinesen_US
dc.subject.meshSalmonella typhi--immunologyen_US
dc.subject.meshT-Cell Antigen Specificityen_US
dc.titleUse of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cellsen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pntd.0005912
dc.identifier.ispublishedNoen_US
dc.description.urinamePLOS Neglected Tropical Diseasesen_US
refterms.dateFOA2019-02-19T18:28:59Z


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